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    Experiment 2: Plant Tissues: Biology Sb015 Lab E-Report

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    RANIA TEONI BINTI TEO KO SIONG Moe
    This document contains an experiment report on plant tissues from a biology unit. The objectives were to prepare an onion epidermal cell slide and identify its structures. Procedures included stripping onion epidermal layers, applying iodine, and observing under microscopes. Structures like the cell wall, nucleus, and cytoplasm were labeled in a drawing. The discussion explains parenchyma and collenchyma tissues and their functions. The objectives were achieved by observing cell structures under magnification.

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    Experiment 2: Plant Tissues: Biology Sb015 Lab E-Report

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    RANIA TEONI BINTI TEO KO SIONG Moe
    8K views5 pages
    This document contains an experiment report on plant tissues from a biology unit. The objectives were to prepare an onion epidermal cell slide and identify its structures. Procedures included stripping onion epidermal layers, applying iodine, and observing under microscopes. Structures like the cell wall, nucleus, and cytoplasm were labeled in a drawing. The discussion explains parenchyma and collenchyma tissues and their functions. The objectives were achieved by observing cell structures under magnification.

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    This document contains an experiment report on plant tissues from a biology unit. The objectives were to prepare an onion epidermal cell slide and identify its structures. Procedures included stripping onion epidermal layers, applying iodine, and observing under microscopes. Structures like the cell wall, nucleus, and cytoplasm were labeled in a drawing. The discussion explains parenchyma and collenchyma tissues and their functions. The objectives were achieved by observing cell structures under magnification.

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    © All Rights Reserved
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    8K views5 pages

    Experiment 2: Plant Tissues: Biology Sb015 Lab E-Report

    Uploaded by

    RANIA TEONI BINTI TEO KO SIONG Moe
    This document contains an experiment report on plant tissues from a biology unit. The objectives were to prepare an onion epidermal cell slide and identify its structures. Procedures included stripping onion epidermal layers, applying iodine, and observing under microscopes. Structures like the cell wall, nucleus, and cytoplasm were labeled in a drawing. The discussion explains parenchyma and collenchyma tissues and their functions. The objectives were achieved by observing cell structures under magnification.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
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    of 5

    BIOLOGY UNIT

    LABUAN MATRICULATION COLLEGE


    BATCH 2022/2023

    BIOLOGY SB015
    LAB E-REPORT

    EXPERIMENT 2: PLANT TISSUES

    NAME: RANIA TEONI BINTI TEO KO SIONG


    MATRIC NUMBER: MA 2215109276
    TUTORIAL CLASS: H04
    LECTURER’S NAME: MOHAMAD TARMIZI BIN ISMAIL
    Experiment: Plant tissue

    Objectives:
    I) To prepare an onion epidermal cell slide.
    II) To identify the structure off the onion epidermal cell

    Introduction:
    Plant tissues are divided into two types; meristematic tissues and permanent
    tissues. Permanent tissues consists of mature cells that have specialized
    structure and functions. Permanent tissues can be divided into three which
    are dermal tissues (epidermal and peridermal), ground tissues (parenchyma,
    sclerenchyma, collenchyma and endodermis) and vascular tissues (xylem and
    phloem).

    Apparatus:
    Compound microscope, razor blade/ cutter, slide, cover slip, dropper, petri
    dish, dissecting pin, filter paper/tissue paper, forceps, white tiles

    Materials:
    Onion, 1% iodine solution
    (Caution: Be careful while handling razor blade/cutter and chemicals)

    Procedures:
    1. The slide was cleaned from any dirt.
    2. The onion was cut into small pieces.
    3. The inner epidermal layer was stripped off from the scale of an onion and
    place it on the slide.
    4. 1-2 drops of dilute iodine solution was applied on the epidermal layer.
    5. The cover slip was lowered carefully by using pin/pen.
    6. Tissue/ filter paper was used to absorb excess iodine on the slide.
    7. The epidermal tissues was observed using 10x and 40x objective lens
    8. The cell wall, nucleus, and cytoplasm were drawn and labelled using 10x
    or 40x objective lens.
    9. The waste was disposed in the bin provided and the apparatus was
    cleaned.
    Results and observation:
    Title: Onion Epidermal Cell

    Actual magnification: 400x


    Discussion:
    Explain the structures, distribution and functions of parenchyma and
    collenchyma.

    Parenchyma:

    The primary cell wall is very thin and flexible. The secondary cell wall is
    absent. There is a present of large vacuole at the centre of the cell. There is a
    present of gap between the cell called intercellular cell. The cell are alive at
    maturity and retain the ability to divide. The distribution of parenchyma is at
    the large centre vacuole. The presence of intercellular space enables the
    tissue to repair wound.

    Collenchyma:

    The primary cell wall of collenchyma is unevenly thickened especially at the


    corner giving rise to polygonal appearance. The secondary cell wall is absent.
    The intercellular space is absent. The large centre vacuole is absent. The
    cells are alive at maturity. The distribution of collenchyma is at peripheral
    region of stems and leaves. The slightly thickened at cell wall enables these
    tissue tissue to provide strong mechanical but flexible support to the plant cell.

    Conclusion:
    The objective at the experiment were achieved. Cytoplasm, cell wall and
    nucleus can be observed. Cytoplasm, cell wall and nucleus can be observed
    by using 10x and 40x objective lens. Cell wall has a fixed shape. This is due
    to the present of cellulose.

    References: (according to APA system)

    Urry, L. A., Cain, M. L., Wasserman, S. A., Minorsky, P. V., Orr, R.B.(2-21).
    Campbell Biology. (12th edition). Pearson Education.

    Questions:
    a) Why do onion epidermal cells contain no chloroplast?

    Because onion epidermal cells does not carry put photosynthesis.

    b) Why are onion cells tightly packed together?

    To provide strength and support as well as maximise the amount of materials


    being stored in onion epidermal cell.

    c) Why is it essential to place the coverslip gently?

    To avoid the entry of air bubbles on the slide

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