Bioprocess Principles & Ipc QP

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BIOPROCESS PRINCIPLES (TBE503)

B.Tech. 3rd Year 5th Semester (Session: 20015-16)


Biochemical Engineering
Time: 2hrs] [Max Marks: 50
Note: Attempt all questions. Assume the missing data if any.

1. Attempt any two questions: (5x2=10)


a) How will you define the energy level of the cell? What is the effect of energy level of a
cell on metabolic reactions?
b) Describe the metabolic pathway for the consumption of glucose when ribose sugar and
NADPH both are required in excess.
c) Describe the key crossroad for the utilization of carbohydrates, fats and proteins?

2. Attempt any two questions: (5x2=10)


a) What is the regulatory strategy behind the diauxic growth phenomenon?
b) What are constitutive and induced enzymes? With the help of suitable example explain
the induction phenomenon.
c) What is feedback inhibition? What are different types of feedback inhibition found an
amino acid synthesis?

3. Attempt any two questions: (5x2=10)


a) What is allosteric enzyme? Describe the hill equation for allosteric enzyme.
b) An inhibitor (I) is added to the enzymatic reaction at a level of 1.0 g/l. The following data
were obtained. (Km = 9.2 g /l)
V (g/l-min) 0.909 0.658 0.493 0.40 0.333 0.289 0.227
S (g/l) 20 10 6.67 5 4 3.33 2.5
Is the inhibitor competitive or non-competitive?
c) What is mixed inhibitions? Discuss the enzyme kinetics for competitive inhibition.

4. Attempt any two questions: (5x2=10)


a) Define the terms growth energy and maintenance energy. What are major activities of
cell under maintenance activity?
b) Differentiate between primary and secondary metabolites with suitable examples.
c) What are zymogens? Describe the activation of chymotrypsinogen.

5. Attempt any two questions: (5x2=10)


a) Define the terms yield, Degree of reduction and respiration qutient. Differentiate between
instantaneous and overall yield.
b) Determine the amount of (NH4)2SO4 to be supplied in a fermentation medium where the
final cell concentration is 30 g/l in a 103 l culture volume. Assume that the cells have
12% nitrogen by weight and (NH4)2SO4 is the only nitrogen source.
c) Aerobic degradation of benzoic acid by a mixed culture of microorganism can be
represented by the following reaction
C6H5COOH + aO2 + bNH3 → cC5H7NO2 +dH2O +eCO2
(i) Determine a, b, c, d and e if RQ=0.9
(ii) Determine the yield coefficient YX/S and YX/O2.
(iii) Determine degree of reduction for the substrate and bacteria.

INSTRUMENTATION AND PROCESS CONTROL (TCH702)


B.Tech. 4th Year 7th Semester (Session: 2015-16)
Biochemical Engineering
Time: 3hrs] [Max Marks: 100
Note: Attempt all questions. Assume the missing data if any.

1. Attempt any four questions: (5x4=20)


a) Draw the block diagram of a measuring instrument and explain its major elements.
b) What are the phenomenons contributing to the dead zone?
c) Differentiate between precision and accuracy.
d) What are human errors? What are different types of human errors?
e) Define the terms fidelity and dynamic error?
2. Attempt any four questions: (5x4=20)
a) What are the methods used for level measurement?
b) Describe the principle of mass spectroscopy?
c) Describe the construction and principle of pH meter.
d) Determine the flow velocity of water of density 1000 kg/m3 at the head of a pitot tube if it
produces a pressure differential of 10kPa between the outlets.
e) What is beer lamberts law? How will you determine the composition using this principle?
3. Attempt any four questions: (5x4=20)
a) Solve the linear differential equation using Laplace transform:
d2x/dt2 +2 dx/dt + x = u(t) provided x(0) = 0and x’(0) = 0
b) Find the Laplace transform of nth order differential of function f(t).
c) Differentiate between servo and regulatory problem?
d) What is feedback control system? Describe its physical elements.
e) Define impulse function. Find the Laplace transform of impulse function.
4. Attempt any two questions: (10x2=20)
a) Consider the continuously stirred-tank reactor. The reaction occurring is A → B and it proceeds at
a rate r = kC0. Assuming constant density and constant volume V, derive the transfer function
relating the concentration in the reactor to the feed-stream concentration.
b) A step change of magnitude 4 is introduced into a system having the transfer function
Y(s)/X(s) = 10/ (s2 +1.6 s +4)
Determine:
(i) Percent overshoot
(ii) Rise time
(iii) Maximum value of Y(t)
(iv) Ultimate value of Y(t)
c) Write short notes on:
(i) Linearization (ii) Transportation Lag
5. Attempt any two questions: (10x2=20)
a) What are various modes of control action commonly used? Compare the performance of these
controllers for the close loop control system.
b) Describe the response of first order system for P controller in feedback control system for step
change in load. Mention the transfer functions for different elements.
c) A pneumatic proportional controller is used in the process to control the cold stream outlet
temperature within the range of 60 to 120 ̊ F. The controller gain is adjusted so that the output
pressure goes from 3 psig (valve fully closed) to 15 psig (valve fully open) as the measured
temperature goes from 71 to 75 ̊ F with the set point held constant. Find the controller gain Kc.

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