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    Fish Pituitary Extraction: ZOOL-707 3 (2-2) Comparative Endocrinology

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    Iffi
    The document describes two methods for extracting the pituitary gland from fish. The pituitary gland is a small endocrine gland located in the brain. In the first method, a portion of the scalp is dissected and removed to expose the brain and pituitary gland. In the second method, the pituitary gland is accessed through the foramen magnum in the skull. Both methods involve removing tissue from around the brain to expose the pituitary gland, which is then lifted out intact using forceps.

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    Fish Pituitary Extraction: ZOOL-707 3 (2-2) Comparative Endocrinology

    Uploaded by

    Iffi
    16 views8 pages
    The document describes two methods for extracting the pituitary gland from fish. The pituitary gland is a small endocrine gland located in the brain. In the first method, a portion of the scalp is dissected and removed to expose the brain and pituitary gland. In the second method, the pituitary gland is accessed through the foramen magnum in the skull. Both methods involve removing tissue from around the brain to expose the pituitary gland, which is then lifted out intact using forceps.

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    3.3 Fish Pituitary Gland

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    The document describes two methods for extracting the pituitary gland from fish. The pituitary gland is a small endocrine gland located in the brain. In the first method, a portion of the scalp is dissected and removed to expose the brain and pituitary gland. In the second method, the pituitary gland is accessed through the foramen magnum in the skull. Both methods involve removing tissue from around the brain to expose the pituitary gland, which is then lifted out intact using forceps.

    Copyright:

    © All Rights Reserved
    Download as PPTX, PDF, TXT or read online from Scribd
    Download as pptx, pdf, or txt
    16 views8 pages

    Fish Pituitary Extraction: ZOOL-707 3 (2-2) Comparative Endocrinology

    Uploaded by

    Iffi
    The document describes two methods for extracting the pituitary gland from fish. The pituitary gland is a small endocrine gland located in the brain. In the first method, a portion of the scalp is dissected and removed to expose the brain and pituitary gland. In the second method, the pituitary gland is accessed through the foramen magnum in the skull. Both methods involve removing tissue from around the brain to expose the pituitary gland, which is then lifted out intact using forceps.

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    Fish Pituitary Extraction

    ZOOL-707 3(2-2)
    Comparative Endocrinology
    Structure: 

    • Pituitary gland is an endocrine gland situated on the ventral side of the


    brain

    • It is a small, soft, whitish body whose size and shape vary with species

    • It is more or less round in carps; oval in catla and rohu and pear-shaped in
    mrigal

    • The pituitary is located in a concave cavity known as Sella turcica and


    enclosed by by a thin membrane known as durameter

    • It may be attached to the brain by a short stalk called the Infundibular


    stalk.
    Types of pituitary glands

    • Based on the presence or absence of the stalk, the pituitary is classified into
    Leptobasic (with stalk) and Platybasic (without stalk) pituitary

    • Leptobasic pituitary – eg. Carps and catfishes

    • Platybasic pituitary – eg. Glassfish (Ambasis spp.)

    • The teleost pituitary comprises of two parts – the glandular part (the
    adenohypophysis) and the nervous part (the neurohypophysis)
    Collection of pituitary gland:

    • Fish pituitary gland can be collected by dissecting and removing a portion of the
    scalp or through the Foramen magnum
     
    (1) Dissecting and removing a portion of the scalp :
     
    • In this method, the brain case (cranium) is obliquely cut using a butcher’s
    knife/hand saw/bone cutter and the scalp removed
    • The brain is then exposed by removing grey matter and fatty substance with
    foreceps and cotton

    Figure: Making an oblique cut in the cranium Figure: Fatty tissue and grey matter exposed
    • The anterior end (optic and olfactory nerves) of the brain is cut and the entire
    brain is lifted up and laid back, thus exposing the pituitary under a membrane

    • After removing the membrane and the fluid, the pituitary is lifted up by inserting
    the blunt end of the foreceps and carefully transferred to a vial containing a
    preservative

    Figure: The pituitary mounted on


    Figure: The brain being exposed Figure: The pituitary seen as a small whitish body
    to a hand
    (2) Through the Foramen magnum: 

    • Foramen magnum is a large posterior aperture of the skull through which the
    spinal cord passes

    • The grey matter and fatty substance are first removed with the help of foreceps
    and cotton (they are pulled out posteriorly)

    • The brain is then exposed

    • The anterior end (optic and olfactory nerves) of the brain is cut and the entire
    brain is lifted up and laid back, thus exposing the pituitary
    • After removing the fluid the membrane, the pituitary is lifted up by inserting the
    blunt end of the foreceps and carefully transferred to a vial containing a
    preservative

    • The first method is commonly practiced even though the second method is less
    time consuming and a large number of glands can be collected within a short
    time, with a good resale value of the fish

    • Even an untrained person can practise the first method

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