This protocol describes an ex vivo feeder-free culture method to differentiate genetically engineered hematopoietic stem and progenitor cells into allogeneic CAR-NKT cells for off-the-shelf cancer immunotherapy.

This protocol outlines a cost-effective and rapid protein sample pre-fractionation strategy based on highly efficient passive elution from gels, recovering intact proteins for top-down proteomics or partially digested proteins for middle-down proteomics.

Three-dimensional Sn²⁺ perovskite systems with high charge transport efficiency display state-of-the-art thin-film transistor performance for optoelectronic and electronic devices.

The spatial distribution of glycosylated RNAs in single cells can be imaged using a protocol that uses an aptamer and a DNA probe that are specific for sialic acid-containing glycosylated RNAs. It can be applied to imaging other glycosylated RNAs using aptamers for other glycans.

A high-resolution microfabricated device that enables longitudinal measurement of cell force, matrix stiffness and the application of mechanical tension or compression to cells in self-assembled 3D tissues.

Fibertrodes enable the integration of optical and electrical signals in a tapered optical fiber-based implantable neural probe for optogenetic stimulation and simultaneous artifact-free electrophysiological recordings in mice.

This protocol describes the use of photothermal nanoparticles embedded in electrospun nanofibers for gentle laser-mediated photoporation of macromolecules into a variety of cell types and outlines strategies to optimize payload delivery.

Aligning trapped ion mobility with a mass-selective quadrupole and time-of-flight mass spectrometry enables parallel accumulation–serial fragmentation, which improves proteomic analysis. This protocol comprehensively describes PASEF workflows.

This protocol describes an ex vivo feeder-free culture method to differentiate genetically engineered hematopoietic stem and progenitor cells into allogeneic CAR-NKT cells for off-the-shelf cancer immunotherapy.

This protocol outlines a cost-effective and rapid protein sample pre-fractionation strategy based on highly efficient passive elution from gels, recovering intact proteins for top-down proteomics or partially digested proteins for middle-down proteomics.

Three-dimensional Sn²⁺ perovskite systems with high charge transport efficiency display state-of-the-art thin-film transistor performance for optoelectronic and electronic devices.

Microtoroid resonators, when paired with noise reduction via the frequency locked whispering evanescent resonator technique, can detect zeptomolar concentrations and single molecules. This protocol details the fabrication, functionalization and use of the frequency locked whispering evanescent resonator sensing system.

An approach to collect lymph drained from the primary tumor in mice enables the downstream characterization of the samples using metabolomics, lipidomics and flow cytometry.