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Plants are very useful to mankind.

Many of them are used exclusively for
medicinal purposes. According to the
World Health Organization (WHO), “a
medicinal plant is a plant which, in one
or more of its organs, contains
substances that can be used for
therapeutic purposes, or which are
precursors for chemo-pharmaceutical
semi-synthesis.” Such plants are in
great demand by pharmaceutical
companies for their active ingredients.
[1,2]
Diabetes mellitus is one of the most
common disorders affecting almost 6%
of the world population and the
dynamics of the diabetes are changing
rapidly in low- to middle-income
countries.[3] According to International
Diabetes Federation's (IDF) estimates,
80% of the world diabetic population
will be from low- and middle-
income countries in 2030. As per IDF
2011 report, China, India, and the
United States of America have a
diabetic
population of 90.0, 61.3, and 23.7
million, which may be increased up to
129.7, 101.2, and 29.3 million,
respectively, in 2030.[4] Globally,
diabetes is one of the six major causes
of death and also causing various
systemic complications. Diabetes
mellitus is treated by hormone therapy
(insulin) or by administering glucose-
lowering agents such as alpha-
glucosidase inhibitors, sulfonylureas,
biguanides, and thiazolidinediones.
Development of an adverse event is
one of the complications in the
treatment of any systemic disorder;
hence,
many of the research institutes and
pharmaceutical companies are
involved in drug development to find
the
molecules with good therapeutic
potential and less adverse events.[5] In
the USA, 10-25% of patients
experience
an adverse drug reaction and these
adverse drug reactions are responsible
for 3.4-7.0% of hospital admissions.[6]
In traditional systems of medicine,
many plants have been documented to
be useful for the treatment of various
systemic disorders. Many of the
traditional/indigenous systems of
medicine are effective than the
modern system
of medicine, but they suffer from lack
of complete standardization which is
one of the important challenges faced
by the traditional system of medicine.
The concept of polyhedral formulation
is well documented in the ancient
literature. Compared to the single
herb, the polyherbal formulation has
better and extended therapeutic
potential.
Hence, the present study was planned
to formulate and standardize a
polyherbal formulation using a plant
having
known antidiabetic activity and
evaluate its therapeutic effects in
rodents.
MATERIALS AND METHODS
Collection of the plant
Taxonomically identified stem bark of
Glycosmis pentaphylla (Rutaceae),
whole plant of Tridax procumbens
(Asteraceae), and leaves of Mangifera
indica (Anacardiaceae) were collected
from the Alagar kovil region,
Madurai district. The collected plants
were authenticated at the Department
of Botany, American college,
Madurai, Tamil Nadu. The voucher
specimen of the plant was deposited in
the Department of Pharmacology,
Ultra College of Pharmacy, Madurai,
India for further reference.
Animals
Adult Wistar rats (180 ± 10 g) of either
sex were obtained from Sainath
Enterprises, Hyderabad, India. The
animals were housed in large, spacious
polyacrylic cages at an ambient room
temperature with 12-h light/12-h
dark cycle. Rats had free access to
water and rodent pellets diet
(Hindustan Lever Ltd, Bangalore,
India). The
study was approved by the Institute
Animal Ethics Committee of the Ultra
College of Pharmacy and all the
animal experiments were carried out
according to the Committee for the
Purpose of Control and Supervision of
Experiments on Animals (CPCSEA)
guidelines, Ministry of Environment
and Forests, Government of India
Go to:
Plants are very useful to mankind.
Many of them are used exclusively for
medicinal purposes. According to the
World Health Organization (WHO), “a
medicinal plant is a plant which, in one
or more of its organs, contains
substances that can be used for
therapeutic purposes, or which are
precursors for chemo-pharmaceutical
semi-synthesis.” Such plants are in
great demand by pharmaceutical
companies for their active ingredients.
[1,2]
Diabetes mellitus is one of the most
common disorders affecting almost 6%
of the world population and the
dynamics of the diabetes are changing
rapidly in low- to middle-income
countries.[3] According to International
Diabetes Federation's (IDF) estimates,
80% of the world diabetic population
will be from low- and middle-
income countries in 2030. As per IDF
2011 report, China, India, and the
United States of America have a
diabetic
population of 90.0, 61.3, and 23.7
million, which may be increased up to
129.7, 101.2, and 29.3 million,
respectively, in 2030.[4] Globally,
diabetes is one of the six major causes
of death and also causing various
systemic complications. Diabetes
mellitus is treated by hormone therapy
(insulin) or by administering glucose-
lowering agents such as alpha-
glucosidase inhibitors, sulfonylureas,
biguanides, and thiazolidinediones.
Development of an adverse event is
one of the complications in the
treatment of any systemic disorder;
hence,
many of the research institutes and
pharmaceutical companies are
involved in drug development to find
the
molecules with good therapeutic
potential and less adverse events.[5] In
the USA, 10-25% of patients
experience
an adverse drug reaction and these
adverse drug reactions are responsible
for 3.4-7.0% of hospital admissions.[6]
In traditional systems of medicine,
many plants have been documented to
be useful for the treatment of various
systemic disorders. Many of the
traditional/indigenous systems of
medicine are effective than the
modern system
of medicine, but they suffer from lack
of complete standardization which is
one of the important challenges faced
by the traditional system of medicine.
The concept of polyhedral formulation
is well documented in the ancient
literature. Compared to the single
herb, the polyherbal formulation has
better and extended therapeutic
potential.
Hence, the present study was planned
to formulate and standardize a
polyherbal formulation using a plant
having
known antidiabetic activity and
evaluate its therapeutic effects in
rodents.
MATERIALS AND METHODS
Collection of the plant
Taxonomically identified stem bark of
Glycosmis pentaphylla (Rutaceae),
whole plant of Tridax procumbens
(Asteraceae), and leaves of Mangifera
indica (Anacardiaceae) were collected
from the Alagar kovil region,
Madurai district. The collected plants
were authenticated at the Department
of Botany, American college,
Madurai, Tamil Nadu. The voucher
specimen of the plant was deposited in
the Department of Pharmacology,
Ultra College of Pharmacy, Madurai,
India for further reference.
Animals
Adult Wistar rats (180 ± 10 g) of either
sex were obtained from Sainath
Enterprises, Hyderabad, India. The
animals were housed in large, spacious
polyacrylic cages at an ambient room
temperature with 12-h light/12-h
dark cycle. Rats had free access to
water and rodent pellets diet
(Hindustan Lever Ltd, Bangalore,
India). The
study was approved by the Institute
Animal Ethics Committee of the Ultra
College of Pharmacy and all the
animal experiments were carried out
according to the Committee for the
Purpose of Control and Supervision of
Experiments on Animals (CPCSEA)
guidelines, Ministry of Environment
and Forests, Government of India
Go to:
Plants are very useful to mankind.
Many of them are used exclusively for
medicinal purposes. According to the
World Health Organization (WHO), “a
medicinal plant is a plant which, in one
or more of its organs, contains
substances that can be used for
therapeutic purposes, or which are
precursors for chemo-pharmaceutical
semi-synthesis.” Such plants are in
great demand by pharmaceutical
companies for their active ingredients.
[1,2]
Diabetes mellitus is one of the most
common disorders affecting almost 6%
of the world population and the
dynamics of the diabetes are changing
rapidly in low- to middle-income
countries.[3] According to International
Diabetes Federation's (IDF) estimates,
80% of the world diabetic population
will be from low- and middle-
income countries in 2030. As per IDF
2011 report, China, India, and the
United States of America have a
diabetic
population of 90.0, 61.3, and 23.7
million, which may be increased up to
129.7, 101.2, and 29.3 million,
respectively, in 2030.[4] Globally,
diabetes is one of the six major causes
of death and also causing various
systemic complications. Diabetes
mellitus is treated by hormone therapy
(insulin) or by administering glucose-
lowering agents such as alpha-
glucosidase inhibitors, sulfonylureas,
biguanides, and thiazolidinediones.
Development of an adverse event is
one of the complications in the
treatment of any systemic disorder;
hence,
many of the research institutes and
pharmaceutical companies are
involved in drug development to find
the
molecules with good therapeutic
potential and less adverse events.[5] In
the USA, 10-25% of patients
experience
an adverse drug reaction and these
adverse drug reactions are responsible
for 3.4-7.0% of hospital admissions.[6]
In traditional systems of medicine,
many plants have been documented to
be useful for the treatment of various
systemic disorders. Many of the
traditional/indigenous systems of
medicine are effective than the
modern system
of medicine, but they suffer from lack
of complete standardization which is
one of the important challenges faced
by the traditional system of medicine.
The concept of polyhedral formulation
is well documented in the ancient
literature. Compared to the single
herb, the polyherbal formulation has
better and extended therapeutic
potential.
Hence, the present study was planned
to formulate and standardize a
polyherbal formulation using a plant
having
known antidiabetic activity and
evaluate its therapeutic effects in
rodents.
MATERIALS AND METHODS
Collection of the plant
Taxonomically identified stem bark of
Glycosmis pentaphylla (Rutaceae),
whole plant of Tridax procumbens
(Asteraceae), and leaves of Mangifera
indica (Anacardiaceae) were collected
from the Alagar kovil region,
Madurai district. The collected plants
were authenticated at the Department
of Botany, American college,
Madurai, Tamil Nadu. The voucher
specimen of the plant was deposited in
the Department of Pharmacology,
Ultra College of Pharmacy, Madurai,
India for further reference.
Animals
Adult Wistar rats (180 ± 10 g) of either
sex were obtained from Sainath
Enterprises, Hyderabad, India. The
animals were housed in large, spacious
polyacrylic cages at an ambient room
temperature with 12-h light/12-h
dark cycle. Rats had free access to
water and rodent pellets diet
(Hindustan Lever Ltd, Bangalore,
India). The
study was approved by the Institute
Animal Ethics Committee of the Ultra
College of Pharmacy and all the
animal experiments were carried out
according to the Committee for the
Purpose of Control and Supervision of
Experiments on Animals (CPCSEA)
guidelines, Ministry of Environment
and Forests, Government of India
Go to:
Plants are very useful to mankind.
Many of them are used exclusively for
medicinal purposes. According to the
World Health Organization (WHO), “a
medicinal plant is a plant which, in one
or more of its organs, contains
substances that can be used for
therapeutic purposes, or which are
precursors for chemo-pharmaceutical
semi-synthesis.” Such plants are in
great demand by pharmaceutical
companies for their active ingredients.
[1,2]
Diabetes mellitus is one of the most
common disorders affecting almost 6%
of the world population and the
dynamics of the diabetes are changing
rapidly in low- to middle-income
countries.[3] According to International
Diabetes Federation's (IDF) estimates,
80% of the world diabetic population
will be from low- and middle-
income countries in 2030. As per IDF
2011 report, China, India, and the
United States of America have a
diabetic
population of 90.0, 61.3, and 23.7
million, which may be increased up to
129.7, 101.2, and 29.3 million,
respectively, in 2030.[4] Globally,
diabetes is one of the six major causes
of death and also causing various
systemic complications. Diabetes
mellitus is treated by hormone therapy
(insulin) or by administering glucose-
lowering agents such as alpha-
glucosidase inhibitors, sulfonylureas,
biguanides, and thiazolidinediones.
Development of an adverse event is
one of the complications in the
treatment of any systemic disorder;
hence,
many of the research institutes and
pharmaceutical companies are
involved in drug development to find
the
molecules with good therapeutic
potential and less adverse events.[5] In
the USA, 10-25% of patients
experience
an adverse drug reaction and these
adverse drug reactions are responsible
for 3.4-7.0% of hospital admissions.[6]
In traditional systems of medicine,
many plants have been documented to
be useful for the treatment of various
systemic disorders. Many of the
traditional/indigenous systems of
medicine are effective than the
modern system
of medicine, but they suffer from lack
of complete standardization which is
one of the important challenges faced
by the traditional system of medicine.
The concept of polyhedral formulation
is well documented in the ancient
literature. Compared to the single
herb, the polyherbal formulation has
better and extended therapeutic
potential.
Hence, the present study was planned
to formulate and standardize a
polyherbal formulation using a plant
having
known antidiabetic activity and
evaluate its therapeutic effects in
rodents.
MATERIALS AND METHODS
Collection of the plant
Taxonomically identified stem bark of
Glycosmis pentaphylla (Rutaceae),
whole plant of Tridax procumbens
(Asteraceae), and leaves of Mangifera
indica (Anacardiaceae) were collected
from the Alagar kovil region,
Madurai district. The collected plants
were authenticated at the Department
of Botany, American college,
Madurai, Tamil Nadu. The voucher
specimen of the plant was deposited in
the Department of Pharmacology,
Ultra College of Pharmacy, Madurai,
India for further reference.
Animals
Adult Wistar rats (180 ± 10 g) of either
sex were obtained from Sainath
Enterprises, Hyderabad, India. The
animals were housed in large, spacious
polyacrylic cages at an ambient room
temperature with 12-h light/12-h
dark cycle. Rats had free access to
water and rodent pellets diet
(Hindustan Lever Ltd, Bangalore,
India). The
study was approved by the Institute
Animal Ethics Committee of the Ultra
College of Pharmacy and all the
animal experiments were carried out
according to the Committee for the
Purpose of Control and Supervision of
Experiments on Animals (CPCSEA)
guidelines, Ministry of Environment
and Forests, Government of India
Go to:
Plants are very useful to mankind.
Many of them are used exclusively for
medicinal purposes. According to the
World Health Organization (WHO), “a
medicinal plant is a plant which, in one
or more of its organs, contains
substances that can be used for
therapeutic purposes, or which are
precursors for chemo-pharmaceutical
semi-synthesis.” Such plants are in
great demand by pharmaceutical
companies for their active ingredients.
[1,2]
Diabetes mellitus is one of the most
common disorders affecting almost 6%
of the world population and the
dynamics of the diabetes are changing
rapidly in low- to middle-income
countries.[3] According to International
Diabetes Federation's (IDF) estimates,
80% of the world diabetic population
will be from low- and middle-
income countries in 2030. As per IDF
2011 report, China, India, and the
United States of America have a
diabetic
population of 90.0, 61.3, and 23.7
million, which may be increased up to
129.7, 101.2, and 29.3 million,
respectively, in 2030.[4] Globally,
diabetes is one of the six major causes
of death and also causing various
systemic complications. Diabetes
mellitus is treated by hormone therapy
(insulin) or by administering glucose-
lowering agents such as alpha-
glucosidase inhibitors, sulfonylureas,
biguanides, and thiazolidinediones.
Development of an adverse event is
one of the complications in the
treatment of any systemic disorder;
hence,
many of the research institutes and
pharmaceutical companies are
involved in drug development to find
the
molecules with good therapeutic
potential and less adverse events.[5] In
the USA, 10-25% of patients
experience
an adverse drug reaction and these
adverse drug reactions are responsible
for 3.4-7.0% of hospital admissions.[6]
In traditional systems of medicine,
many plants have been documented to
be useful for the treatment of various
systemic disorders. Many of the
traditional/indigenous systems of
medicine are effective than the
modern system
of medicine, but they suffer from lack
of complete standardization which is
one of the important challenges faced
by the traditional system of medicine.
The concept of polyhedral formulation
is well documented in the ancient
literature. Compared to the single
herb, the polyherbal formulation has
better and extended therapeutic
potential.
Hence, the present study was planned
to formulate and standardize a
polyherbal formulation using a plant
having
known antidiabetic activity and
evaluate its therapeutic effects in
rodents.
MATERIALS AND METHODS
Collection of the plant
Taxonomically identified stem bark of
Glycosmis pentaphylla (Rutaceae),
whole plant of Tridax procumbens
(Asteraceae), and leaves of Mangifera
indica (Anacardiaceae) were collected
from the Alagar kovil region,
Madurai district. The collected plants
were authenticated at the Department
of Botany, American college,
Madurai, Tamil Nadu. The voucher
specimen of the plant was deposited in
the Department of Pharmacology,
Ultra College of Pharmacy, Madurai,
India for further reference.
Animals
Adult Wistar rats (180 ± 10 g) of either
sex were obtained from Sainath
Enterprises, Hyderabad, India. The
animals were housed in large, spacious
polyacrylic cages at an ambient room
temperature with 12-h light/12-h
dark cycle. Rats had free access to
water and rodent pellets diet
(Hindustan Lever Ltd, Bangalore,
India). The
study was approved by the Institute
Animal Ethics Committee of the Ultra
College of Pharmacy and all the
animal experiments were carried out
according to the Committee for the
Purpose of Control and Supervision of
Experiments on Animals (CPCSEA)
guidelines, Ministry of Environment
and Forests, Government of India
MATERIALS AND METHODS Collection of the plant Taxonomically identified stem bark of Glycosmis
pentaphylla (Rutaceae), whole plant of Tridax procumbens (Asteraceae), and leaves of Mangifera indica
(Anacardiaceae) were collected from the Alagar kovil region, Madurai district. The collected plants were
authenticated at the Department of Botany, American college, Madurai, Tamil Nadu. The voucher
specimen of the plant was deposited in the Department of Pharmacology, Ultra College of Pharmacy,
Madurai, India for further reference.

Animals Adult Wistar rats (180 ± 10 g) of either sex were obtained from Sainath Enterprises, Hyderabad,
India. The animals were housed in large, spacious polyacrylic cages at an ambient room temperature
with 12-h light/12-h dark cycle. Rats had free access to water and rodent pellets diet (Hindustan Lever
Ltd, Bangalore, India). The study was approved by the Institute Animal Ethics Committee of the Sultan Ul
Uloom College of Pharmacy and all the animal experiments were carried out according to the
Committee for the Purpose of Control and Supervision of Experiments on Animals (CPCSEA) guidelines,
Ministry of Environment and Forests, Government of India.

The effect of polyherbal formulation on

fasting blood glucose levels (mg/dl) in STZ-
and NIC-induced diabetic rats

Effect of polyherbal formulation on plasma

insulin level in STZ-and NIC-induced
diabetic rats