BioAir Top Safe - User Manual 2015 pg1-46

Class II Microbiological Safety Cabinets
TopSafe series
1.2 – 1.5 – 1.8
OPERATING MANUAL
PART 1: USER
PART 2: SERVICE AND
MAINTENANCE
ORIGINAL INSTRUCTION
80.021.021 Revision 09 July 2015
EuroClone S.p.A.
Head offices: Via Figino, 20/22 - 20016 Pero (MI) Italy - Tel. +39 02 38195.1 - Fax +39 02 8195250
Mfg. plant: Via Lombardia,12 - 27010 Siziano (PV) Italy - Tel. +39 02 38195.1 - Fax +39 02 38195248
www.euroclone.it - info@euroclone.it
EuroClone S.p.A. has a Quality System certified in compliance with EN ISO 9001:2008 and EN ISO 13485:2012
80.021.021 r09 - July 2015
Topafe series Operating Manual
GENERAL INFORMATION - FOREWORD
This manual is specifically designed to instruct the User and Service in the operation and function
of the following standard TOPSAFE family cabinets:
Model: TOPSAFE
Sizes: 1.2 – 1.5 – 1.8
MAINS SUPPLY AT 230 V~ 50 Hz ONLY

Codes: LDA0000; LDB0000; LDC0000
LDA0300; LDB0300; LDC0300
MAINS SUPPLY AT 220 V~ 60 Hz ONLY

Codes: LDA0060; LDB0060; LDC0060
The manual consists of two parts:

1. Part 1 is specifically designed to instruct the users.
2. Part 2 is specifically designed to train service personnel only.
Instructions listed in this part are not designed to user.
NOTE: service personnel who will operates on this cabinet should be however authorized.
This Manual shall never be parted from the cabinet in order to be quickly available for use,
service, maintenance and routine control.
The instructions given in this Manual shall be carefully read and understood before switching this
equipment on, before any service operations and before you operate it, in order to become
familiar with its operation and function, and to avoid misuse.
While every care has been taken in the production of this manual, it is not designed to act as
your procedure manual or act as a substitute for validation of your own procedures.
EUROCLONE reserves the right to modify or update the content at any time without prior notice.
WARNING!
• This manual refers to the above cabinet codes only.

• The code is printed on the label (see LABELLING).
• Unless otherwise specified, all information are applicable to all above codes.
• This is a basic manual and it must be upgraded in case of different codes.
• Special versions of TOPSAFE cabinet with different code are partially covered by this
manual, but they need additional instruction to add or substitute parts of this manual.
• In cases of special cabinets, please read also the additional user manual
referred to relevant cabinet code.
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If you require any further information or explanation of the information contained in this manual,
and for any request relevant to
• service
• spare parts
• technical enquiries
contact:
WITHIN ITALY ABROAD

SAT Italia
Tel. + 39 02 38195275 contact your local distributor or
Fax + 39 02 38195236
e-mail: sat@euroclone.it e-mail: service@euroclone.it
For any additional information, please contact the manufacturer:
EUROCLONE S.P.A.
Via Figino, 20/22
20016 Pero (MI)
Tel. + 39 02 381951
Fax + 39 02 38101465
e-mail: info@euroclone.it
Check the manufacturer’s label for the code and the serial number of the cabinet.
LABELLING
On the light box of every apparatus a label is placed with the following important information as
shown in the example below (Pic. 1):
Logo
Name of manufacturer
Address of manufacturer
CE Mark (for European Market only)
Model
Code
Voltage, Frequency, Power
Warning (3)
Serial number
Year of manufacturing
Standard conformity (1)
Competent Body Mark (2)
Factory use code (4)
Pic. 1
Do not remove this label.

For any enquiry relevant to service or spares parts, please always refer to the labelling of the
unit and report those data to us.
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MEANING OF SYMBOLS
GENERAL WARNING.
CAREFULLY READ THE MANUAL BEFORE USING.
BIOLOGICAL HAZARD WARNING
UV-C HAZARD WARNING
PROVISIONS SIGN
MAIN GROUND TERMINAL BLOCK
Separated collection for electric and electronic components.

According to EU DIR. 2002/96/EC.
GLOSSARY
Apf: Aperture protection factor.
DOP: Dispersed Oil Particle.
EMC: Electromagnetic Compatibility.
EMI: Electromagnetic Interference.
Exh: Exhaust air flow.
HEPA: High Efficiency Particulate Air.
LAF: Laminar Air Flow.
MPPS: Most Penetrating Particle Size.
MSC: Microbiological Safety Cabinet.
PELV: Protection by Extra-Low Voltage
PFA: Paraformaldehyde.
PPD: Personal Protection Device
UVC: Ultraviolet radiation (range C - germicidal).
VFC: Voltage Free Contact.
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CONTENTS
GENERAL INFORMATION - FOREWORD 3
LABELLING 4
MEANING OF SYMBOLS 5
GLOSSARY 5
PART 1: USER 9
1. ASSESSMENT OF RISKS 11
2. RULING, STANDARDS AND DEFINITION 17
2.1 EC DECLARATION OF CONFORMITY 17
2.2 DECLARATION OF CONFORMITY FOR EXTRA UE MARKETS ONLY 17
2.3 CABINET DEFINITION 18
3. INSTALLATION 19
3.1 CHOICE OF LOCATION 19
3.2 FLOOR STAND 19
3.3 CONNECTING TO THE MAINS 19
3.4 CONNECTING TO THE COMBUSTIBLE GAS LINE 21
3.5 CONNECTING TO THE INERT FLUIDS/VACUUM LINE 21
3.6 FINAL PROCEDURES 21
4. TECHNICAL DATA 22
4.1 SPECIFICATIONS 22
4.2 USE ENVIRONMENTAL CONDITIONS 23
4.3 TRANSPORT AND STORAGE CONDITIONS 23
4.4 WEIGHT AND DIMENSIONS 23
4.5 MATERIALS 23
4.6 PERFORMANCES 24
4.7 FILTERS DIMENSIONS AND FEATURES 24
4.8 STANDARD ELECTRICAL EQUIPMENT 25
4.9 STANDARD UTILITIES 25
4.10 MAIN OPTIONALS 26
5. OPERATING PRINCIPLE 27
6. OPERATING INSTRUCTIONS 28
6.1 CABINET DESCRIPTION 28
6.2 CONTROL PANEL 29
6.3 UTILITIES 32
6.4 FRONT APERTURE 35
6.5 WORKING SURFACE 37
6.6 OPERATING DESCRIPTION 38
6.7 OPERATING PROCEDURE 39
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7. GENERAL RULES – GUIDELINES 44

7.1 FEATURES OF LOCATION: 44
7.2 WORKING POSTURE: 44
7.3 WORKING PROCEDURES: 44
8. CLEANSING AND DECONTAMINATION 46
8.1 CLEANSING 46
8.2 DECONTAMINATION 46
PART 2: SERVICE AND MAINTENANCE 47
9. INSTALLATION 49
9.1 HANDLING AND UNPACKING 49
9.2 POSITIONING 49
9.3 INSTALLATION OF OPTIONAL EQUIPMENT 50
9.4 MAINS CONNECTION 52
9.5 GAS LINE CONNECTIONS 52
9.6 PERFORMANCE TEST 53
10. SERVICE AND MAINTENANCE 54
10.1 PC BOARD SETTING 54
10.2 AIRFLOWS MEASUREMENT METHODS 58
10.3 CALIBRATION OF AUTOMATIC CONTROL 60
10.4 ELECTRIC COMPONENTS LOCATION 63
10.5 FILTERS TEST AND REPLACEMENT 64
10.6 TROUBLE SHOOTING 68
10.7 SPARE PARTS LIST 70
11. DECONTAMINATION 72
11.1 DECONTAMINATION BY FUMIGATION 72
12. WIRING DIAGRAM 78
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TopSafe series Operating Manual
Part 1: User
PART 1:
USER
CABINETS
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Part 1: User
1. ASSESSMENT OF RISKS
TOPSAFE is a Class II Microbiological Safety Cabinet as per EN 12469:2000.
NOTE: notwithstanding conformity with the above standard, during operating of the cabinet
several residual risks remain and were assessed in order to take adequate measures to prevent
the following hazards arising from normal use and foreseeable misuse:
General
Misuse
Electric shock
Biological
UVC radiations
Spread of fire from the equipment
Liberated gases and explosion
The analysis were done as per EN 61010-1:2010, 7.3.3 and Annex J.
The residual risks have been assessed as per the following Tab. 1:
Tab. 1 – Risk Category
CATEGORY ASSESSMENT DESCRIPTION
1 Broadly This Category fulfils the requirement for tolerable risk.
acceptable.
2 As low as This Category does not automatically fulfil the requirement for
reasonably tolerable risk. If possible, reduce these risk to Category 1.
practicable.
If not possible, then the instructions should contain a
description of the risk so that the Responsible Body can take
appropriate steps to protect the safety of operators.
3 Intolerable. This category contains risks that are not tolerable risks.
NOTE: no residual risk of this cabinet belongs to Category 3.
Read the fallowing warnings before operating this cabinet.
Additional warnings shall be shown at the specific paragraphs.
1.1.1 WARNING! General hazard. Risk category: 1

• The cabinet is supplied “NOT READY TO USE”.
• Installation test should be done to assure the correct performance of the cabinet.
• Unpacking, positioning, assembling and connecting to the mains shall be carried out
solely by specialized and authorized staff, following the instructions listed in the Operating
Manual, PART 2 – Service and Maintenance
Taken measures to reduce the risk:
• Warning marking (caution) is provided on the cabinet.
• Adequate information for user.
• DO NOT connect the cabinet to the mains before it has been properly installed.
• DO NOT switch on the system before an installation and performance test
report is issued by a specialized and authorized staff.
• DO NOT operate this cabinet before you have carefully read and understood
this manual.
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Part 1: User
1.1.2 WARNING! Misuse hazard. Risk category: 1

• Misuse or a use which doesn’t comply with the instructions of this manual compromises
the safety of the cabinet.
• Biological hazard for personnel, environment and product.
• The cabinet shall be used by authorized and adequately trained personnel only.
• Read carefully this manual before using the cabinet.
• Keep this manual always and easily available.
• DO NOT allow unauthorized people or general public to approach the cabinet.
• DO NOT leave the removable key in the main switch. Remove it always in both
ON and OFF position and keep it in a safe place.
1.1.3 WARNING! Biological hazard. Risk category: 1

• Biologically safe conditions are ensured when cabinet is on in MODE 1 only.
• Biologically safe conditions are NO longer ensured when audible and/or visible alarm
devices turn on.
• DO NOT work when alarm is on.

When the alarm is on with the glass window in work position:
• Close the front access.
• Shut down the cabinet.
• Call servicing organization.

• The cabinet doesn’t protects neither the operator nor products from contamination
deriving from massive spilling of substances or use of inadequate techniques.
• Wear appropriate clothes, with long sleeves and elastic cuffs, and rubber
gloves.
• Wash hands and arms with a germicidal soap before and after work.
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Part 1: User

• The obstruction of the slots of the front grill may jeopardize the Operators’ safety and the
product protection.
• The intake air grill at the front of the cabinet working surface assures the containment of
the contaminated air inside the cabinet and the protection of the product against
environment contamination.
• Keep always free from any object the slots of the front grill.
• Pay especially attention to paper, plastic film and cloths for cleaning
1.1.7 WARNING! UVC radiations hazard. Risk category: 1

• The front glass windows (made of laminated safety glass) is a protection against UVC
radiations (98%). Moreover its integrity combined with the right position determines the
correct velocity of the intake air barrier through the front aperture.
• The breakage of front glass window compromises both the UVC radiation protection and
the air barrier containment.
• The maximum energy of an impact to which the glass can withstand is 4 J. The test
passed was carried out as per IEC 61010-1, clause 8.2.2, with smooth steel sphere with
mass 500g ±25g in vertical fall from 80 cm on horizontal surface (impact energy= 4 J).
Next step of test (vertical fall from 100 cm – impact energy= 5 J) failed.
• The minimum value of impact energy required is 5 J. Lower protection degree is
acceptable, but not less than 1 J, only if additional protective measures are taken.
• Use by unauthorized people or the general public is not allowed.
• Warning marking (caution) is provided on protective glass.
• DO NOT allow unauthorized people or general public to approach the cabinet.

In case of breakage of the glass:
• DO NOT work.
• NEVER expose skin and eyes to such radiations.
• Switch immediately off UVC function.
• Close the front access.
• Shut down the cabinet.
• Call servicing organization.
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Part 1: User
1.1.8 WARNING! UVC radiations hazard. Risk category: 1

• Direct exposure to UVC radiations may cause erythema and conjunctivitis.
• UVC radiations may generate ozone which may be an inhalation hazard.
• UV radiation causes degradation of certain materials that may be used in cabinet
construction. The breakdown of some materials within the work zone may result in the
emission of toxic vapours.
• The protections measures against UVC radiations hazards don’t cover a misuse of servicer
socket (i.e. as UVC supply). This means that UVC lamp connected to this socket can be lit
up without the protection of the front glass window.
• The supplied UVC lamp complies with IEC/EN 62471.
• The closure of the front glass window contains inside the cabinet at least 98% of UVC
radiations.
• The lighting of UVC lamp is allowed with the front aperture completely closed only.
• The key switch allows to lock the cabinet, preventing unauthorized use.
• Warning marking (caution) is provided on protective glass.
• DO NOT allow unauthorized people or general public to approach the cabinet..

• The use of this cabinet should be allowed to authorized, trained and informed
personnel only.
• DO NOT plug any additional UVC fixture.
• DO NOT use the service socket for any UV fixture.
• Use UVC lamp device recommended by EUROCLONE only.
• DO NOT leave the removable key in the main switch. Remove it always in both
ON and OFF position and keep it in a safe place.
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1.1.9 WARNING! Pinch hazard. Risk category: 1
1.1.10 WARNING! Electric shock hazard. Risk category: 1
• Not accessible parts of front glass window need unlock and swing-up operation, which
allows access to electric and moving components, with pinch risk of body parts and
electric shock risk.
• This operation must be performed by adequately trained and authorized Service

staff only, following instructions of PART 2 of this manual.
1.1.11 WARNING! Spread of fire from the equipment hazard. Risk category: 1
• Use of flame (combustible gas line) is possible during normal use.
• In case of sudden no-airflow condition (i.e. fan failure) the flame may raise up to the
filter, causing serious hazard of ignition.
• 2 ways normally closed solenoid valve (EV gas) on the combustible gas line is installed to
prevent this hazard.
• EV gas is disabled every time no-safe conditions occur, breaking the combustible gas
flow.
• Warning marking (caution) is provided close to the gas tap.
• Use this dedicated combustible gas line only to connect devices for use of
flame.
1.1.12 WARNING! Liberated gases and explosion hazard. Risk category: 1

• Neither accidental blowing out of the flame nor related escaping of gas won’t be detected.
• No gas detection device is installed.
• EV gas doesn’t prevent this kind of hazard.
• Warning marking (caution) is provided close to the gas tap.
• Use Bunsen burner only with flame control or piezoelectric ignition.

• DO NOT connect combustible gas to vacuum/inert gas line.
• DO NOT leave the cabinet with open flames.
• Refer to all guidelines about the use of combustible gas.
• Keep the gas tap closed and EV gas OFF when not in use.
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Part 1: User
1.1.13 WARNING! Liberated gases and explosion hazard. Risk category: 2

• Decontamination of the cabinet is mandatory before any maintenance work, filter
replacement or other operations requiring access to any potentially contaminated section
of the cabinet, before dislocation or after any emergency.
• Cabinet users shall identify the decontaminating method effective against the agent in
question.
• A method of decontamination is described in the Operating Manual, PART 2 - Service.
• Formalin vapours are harmful.
• Formaldehyde vapours are explosive at 75% concentration in dry air.
• Its ignition point is at 430 °C.
• Auto ignition temperature of paraformaldehyde (PFA) is at 300 °C.
• Decontamination shall be carried out solely by specialized and authorized

staff, following the instructions listed in the PART 2 of this manual (Service)
and following manufacturer instructions of manipulate substance.
• DO NOT presence during decontamination activity.
• Individual respiratory outfit should be available on the spot to protect the
personnel against unforeseeable breathing of formalin vapour.
• Consult safety officer for precautions requested by local authorities.
THE MANUFACTURER DECLINES ALL CRIMINAL AND CIVIL LIABILITY FOR DEFECTS DUE TO
MISUSE, UNAUTHORIZED USE, TAMPERING OR ANY GENERAL USE OF THE EQUIPMENT THAT IS
NOT PERMITTED OR THAT DOES NOT COMPLY WITH THE OPERATING INSTRUCTIONS
CONTAINED IN THIS MANUAL.
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Part 1: User
2. RULING, STANDARDS AND DEFINITION
2.1 EC DECLARATION OF CONFORMITY

The Manufacturer:
EuroClone S.p.A.
Via Figino 20/22
20016 Pero (MI) - Italia
HEREWITH DECLARES THAT THE FOLLOWING PRODUCTS:
MODEL: TopSafe
SIZES: 1.2 – 1.5 – 1.8
CODES: LDA0000; LDB0000; LDC0000
LDA0300; LDB0300; LDC0300
DESCRIPTION: CLASS II MICROBIOLOGICAL SAFETY CABINETS
COMPLY WITH THE PROVISIONS OF THE FOLLOWING DIRECTIVES:
2006/42/EC Machinery Directive

2004/108/EC Electromagnetic Compatibility Directive (EMC)
AND THAT THE FOLLOWING STANDARDS HAVE BEEN APPLIED:
EN 61010-1:2010 Safety requirements for electrical equipment for

measurement, control and laboratory use. Part 1: General
requirements.
EN 61326-1:2013 Electrical equipment for measurement, control and

laboratory use - EMC requirements - Part 1: General
requirements.
EN 12469:2000 Biotechnology - Performance criteria for microbiological safety

cabinets.
2.2 DECLARATION OF CONFORMITY FOR EXTRA UE MARKETS ONLY

THE FOLLOWING PRODUCTS (MAINS AT 220 V~ 60 Hz) OF THE SAME FAMILY:
CODES: LDA0060; LDB0060; LDC0060

ARE BUILT FOR EXTRA UE MARKETS ONLY AND COMPLY WITH THE FOLLOWING STANDARDS:
IEC 61010-1:2010 Safety requirements for electrical equipment for

measurement, control and laboratory use. Part 1: General
requirements.
IEC 61326-1:2006 Electrical equipment for measurement, control and
laboratory use - EMC requirements - Part 1: General
requirements.
EN 12469:2000 Biotechnology - Performance criteria for microbiological safety
cabinets.
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Part 1: User
2.3 CABINET DEFINITION
TOPSAFE cabinets are defined as follows:

CLASS II MICROBIOLOGICAL SAFETY CABINET (MSC).
MSC class II as per EN 12469:2000, clause 3.5:
Safety cabinet with a front aperture through which the operator can carry out manipulations
inside the cabinet and which is constructed so that the worker is protected, the risk of
product and cross contamination is low and the escape of airborne particulate contamination
generated within the cabinet is controlled by means of an appropriate filtered internal airflow
and filtration of the exhaust air.
This cabinet is designed to reduce the risk to the operator when handling hazardous or
potentially hazardous microorganisms and to protect the materials being handled in them from
environmental contamination. In such a condition the environment is also protected.
Designed to protect the personnel and the environment against the risks relevant to the handling
of biological agents, and to protect the product against the cross contamination risk.
TOPSAFE cabinet is designed for handling biological agents according to regulations applied in
the users’ countries.
Categories of etiological agents are defined by the statutory legislation in your country.
Reference to the listed classes of microbial species is consequently IMPERATIVE in order to
assess which ones can be handled in this MSC.
TOPSAFE cabinet is designed to recycle the exhaust air into the laboratory.
NOTE 1: changes or different configurations of the cabinet (like adding filters, pre-filters and/or
ducting) invalidate any approval markings obtained.
If an exhaust additional filter or if the exhaust air must be ducted outside the laboratory,
purposely designed accessories (extraction kits) may be supplied with, or for already installed
cabinets.
NOTE 2: should the exhaust air be ducted outside the laboratory, EUROCLONE or local dealers
will assist according to the specific laboratory or building configuration. A list of accessories is
available to equip the cabinets.
WARNING!
• MSCs ducted outside the laboratory require an additional validation by qualified

personnel.
WARNING! See ASSESSMENT OF RISKS, warning 1.1.2
THE MANUFACTURER DECLINES ALL CRIMINAL AND CIVIL LIABILITY FOR DEFECTS
DUE TO MISUSE, UNAUTHORIZED USE, TAMPERING OR ANY GENERAL USE OF THE
EQUIPMENT THAT IS NOT PERMITTED OR THAT DOES NOT COMPLY WITH THE
OPERATING INSTRUCTIONS CONTAINED IN THIS MANUAL.
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Part 1: User
3. INSTALLATION
NOTE: unpacking, positioning, assembling and connecting to the mains shall be carried out
solely by specialized and authorized staff, following the instructions listed in the Operating
Manual, PART 2 – Service and maintenance.
3.1 CHOICE OF LOCATION
WARNING! Biological hazard.
• Not suitable place may compromises the performance and the biological safety of the
cabinet.
• The cabinet shall be installed in an air-draft free room to avoid interference with
the air barrier crossing the front aperture.
• Air conditioning grilles and aisles where foot traffic is intense may have a
negative influence to the cabinet ventilation.
• The distance between the room ceiling and the exhaust air outlet shall be at
least 300 mm.
3.2 FLOOR STAND
WARNING! Instability hazard.
• The cabinet should be fitted on suitable support stand (not supplied) and levelled.
• It is strongly advised the use of dedicated EUROCLONE support stand which is available in
the optional accessories list.
• Use of different accessories is under total responsibility of the customer.
• This operation should be carried out only by qualified service personnel.
3.3 CONNECTING TO THE MAINS

NOTE: this operation should be carried out only by authorized personnel.
The cabinet is designed to operate in the range defined by the table of specifications (see 4.1).
Check the mains supply to match with the one declared on the data plate of the cabinet.
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Part 1: User
3.3.1 Mains socket

A near-based (max 2 m) grounding mains socket shall be available to connect the
cabinet.
Grounding must comply with local regulations.
Check that the mains voltage matches what shown on the manufacturer’s label on the
cabinet.
WARNING!
• The mains plug of the cord is the means for disconnection and it should be visible and
easy-accessible, but not directly from the working position.
• Connect the cabinet to a dedicated power line, with a near-based grounding

socket (max 2 m), according to the length of the power cord (no extension is
allowed), but out of the arms reach of the user (to avoid the cabinet be
switched off accidentally).
• Neither objects nor furniture should hide the plug in order to can quick
disconnect the cabinet in case of emergency.
3.3.2 Mains supply cord

The mains supply cord is a detachable flexible cord, with IEC 60320 C13 female mains
connector and CEE 7/7 mains plug (French-German) moulded at either end (Pic. 2).
It complies with IEC 60227 or IEC 60245 and it is approved by a Competent Body.
WARNING!
• Use of unsuitable mains supply cord compromises the safety of the cabinet.
• Replacement of the mains supply cord (i.e. due to damaged cord or mains plug
not compatible with mains socket), should be done with an approved one only,
with same specifications as per Tab. 2.
Tab. 2 – Mains supply cord specifications

Type of cable: H05VV-F
Numbers of wires: 3
2
Section (mm ): 1,5
Max length (m): 3
Cable conformity: IEC 60799
Mains connector conformity: IEC 60320-1
Mains plug conformity: IEC 60884-1
Approvals: European Pic. 2
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Part 1: User
3.3.3 Mains appliance inlet and main fuses
The appliance inlet (Pic. 3) is placed on the right-

hand compartment top of the cabinet and consists
to a IEC 60320 C14 male connector with a fuse
holder for two main fuses.
The main fuses are replaceable from the outside,

located in the inlet appliance.
Fuses rating are available in the table of

specifications (see 4.1) and marked with a label
near the appliance (Pic. 3).
Pic. 3
3.4 CONNECTING TO THE COMBUSTIBLE GAS LINE

NOTE: this operation should be carried out only by service authorized personnel.
The operating range is defined in the table of specifications (see 4.1).
3.5 CONNECTING TO THE INERT FLUIDS/VACUUM LINE

NOTE: this operation should be carried out only by service authorized personnel.
The operating range is defined in the table of specifications (see 4.1).
3.6 FINAL PROCEDURES
NOTE: Before the first use of the cabinet an efficiency test (installation testing) shall be carried
out as per EN 12469 standard, table 5, and relevant report issued, by specialized and authorized
staff.
Ask your Dealer or EUROCLONE for commissioning.
Once installation is over, carry out the final procedures before operating the cabinet:
make sure that the mains breaker be turned off;
remove the work surface;
carefully clean the cabinet inside and outside following instructions reported at relevant
paragraph (see 8.1);
put the working surface in its position (see 6.5) after checking that no foreign body has
slipped under it (use lint-free clothes);
stick the BIOLOGICAL HAZARD WARNING label (see MEANING OF SYMBOLS) on the
outside part of the door of the room where the cabinet operates.
THE MANUFACTURER DECLINES ALL RESPONSIBILITY FOR DAMAGES DERIVING FROM

INSTALLATION OR ALTERATIONS MADE BY NON QUALIFIED AND/OR NON
AUTHORIZED STAFF.
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Part 1: User
4. TECHNICAL DATA
DESCRIPTION SIZE 1.2 SIZE 1.5 SIZE 1.8
4.1 SPECIFICATIONS
Marks of conformity:
230-240 V~ 50 Hz
| | |
220 V~ 60 Hz (*) NONE NONE NONE
230-240 V~ 50 Hz EN 61010-1:2010 / EN 61326-1:2013 / EN 12469:2000
Reference Standard:
220 V~ 60 Hz (*) IEC 61010-1:2010 / IEC 61326-1:2012 / EN 12469:2000
Electrical insulating/protection class [IEC 61140]: I
230-240 V~ 50 Hz
Mains supply voltage:
(*) 220 V~ 60 Hz
Main fuses rating: F10 A, H (Material: steatite – Size: 5x20 - I2t: 121)
Required power line (W): 230-240 V~ 50 Hz 1300 1450 1600
(3 A service socket included) 220 V~ 60 Hz (*) 1400 1550 1700
Absorbed power (W): 230-240 V~ 50 Hz 465 615 765
(fan and light on only) 220 V~ 60 Hz (*) 565 715 865
Sustained impact maximum energy of the glass
4
[EN 61010-1, clause 8.2.2] (J):
Window glass UVC radiations retention (%): 98
Leaktightness index [EN 12469]: LI-C
Cleanability index [EN 12469]: CI-B
Sterilizability index [EN 12469]: SI-B
Combustible gas fixture max pressure (mbar): 20
Inert fluids/vacuum fixture max pressure (bar): 4
Electrical service socket max current (A): 3
(*) Extra European Market only
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Part 1: User
4.2 USE ENVIRONMENTAL CONDITIONS

Use: indoor
Altitude (m): up to 2000
Temperature (°C): from 10 to 35
Maximum relative humidity (%): 80 for temperatures up to 31 °C, decreasing linearly to 50 at 40 °C
Max MAINS supply voltage fluctuations (%): up to ±10
TRANSIENT OVERVOLTAGE CATEGORY: II
POLLUTION DEGREE: 2
4.3 TRANSPORT AND STORAGE CONDITIONS
Ambient temperature (°C): from -5 to 45
Relative humidity (%): up to 90
Atmospheric pressure (mbar): from 800 to 1060
4.4 WEIGHT AND DIMENSIONS
standard version 233 273 326
Weight (kg):
BS version (**) 243 285 341
Overall dimensions L x D x H (mm):
1380 x 795 x 1450 1685 x 795 x 1450 1990 x 795 x 1450
(without support stand)
Front aperture dimensions L x H (mm) : 1177 x 195 1482 x 195 1787 x 195
Working space dimensions L x D x H (mm): 1230 x 580 x 700 1530 x 580 x 700 1840 x 580 x 700
Safe working area dimensions L x D (mm): 1030 x 350 1335 x 350 1640 x 350
4.5 MATERIALS
Main structure: cold rolled steel, stove enamel coated RAL 7035
Working space surface: stainless steel AISI 304 - 2B finishing
Front window: laminated safety glass
(**) double exhaust filter
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4.6 PERFORMANCES
Laminar Air Flow mean velocity [EN 12469](m/s): 0,36 ÷ 0,40
Inflow Air Barrier mean velocity [EN 12469](m/s): 0,56 ±10%
3
Exhaust Air flow rate (m /h): 460 ±10% 600 ±10% 700 ±10%
Exhaust Air flow ratio (%): 30 ±10
Apf - Aperture Protection Factor [EN 12469]:
≥1,0 x 105
(Retention efficiency at front aperture)
Working space air cleanliness class [EN 14644-1]: ISO 5
Illuminance [EN 12469] (lux): >750
Sound level [EN ISO 3744] (dB[A]): <58 <60 <60
Vibration [EN 12469] (mm RMS): <0,005
Max increase inside cabinet in temperature from the
<5
ambient [EN 12469] (°C):
4.7 FILTERS DIMENSIONS AND FEATURES
LAF filter dimensions L x D x H (mm): 1219 x 610 x 68 1525 x 610 x 68 1829 x 610 x 68
standard version 610 x 457 x 90 610 x 610 x 90 762 x 610 x 90
EXH filter dimensions L x D x H
610 x 457 x 115 610 x 610 x 115 762 x 610 x 115
(mm): BS version (**)
610 x 457 x 115 610 x 610 x 115 762 x 610 x 115
Filters efficiency class [EN 1822-1]: H14
Filters global MPPS efficiency [EN 1822-1](%): 99,995
MPPS diameter [EN1822-1](µm): 0,1 ÷ 0,3
(**) double exhaust filter
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4.8 STANDARD ELECTRICAL EQUIPMENT

Main switch with all position removable key
Automatic electronic airflow velocity control PCB
Motorblower (fan)
2nd motorblower (fan)
Fluorescent lamps
Permanent UV-C lamp
Combustible gas solenoid valve
4.9 STANDARD UTILITIES
Tap for combustible gas line
Tap for inert fluids/vacuum line
Auxiliary electrical service socket
2nd auxiliary electrical service socket optional optional
Mobile UV-C lamp socket
Voltage-free contact (VFC) outlet
Alarm mute connector (for service personnel only)
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CODE DESCRIPTION NOTES SIZE 1.2 SIZE 1.5 SIZE 1.8
4.10 MAIN OPTIONALS

AC10000 CHEST DRAWER 2 drawers – with castors
AS1A000 SUPPORT STAND TS SM 1.2
AS1B000 SUPPORT STAND TS SM 1.5 h= 730 mm
AS1C000 SUPPORT STAND TS SM 1.8
AS1A100 SUPPORT STAND ADJUSTABLE TS SM 1.2
AS1B100 SUPPORT STAND ADJUSTABLE TS SM 1.5 h= 730 mm
AS1C100 SUPPORT STAND ADJUSTABLE TS SM 1.8
AKC0001 KIT UVC VISION 30W (mobile) 230V~50Hz
AKC0061 KIT UVC VISION 30W 60220 (mobile) 220V~60Hz (*)
AZ1A601 ARMRESTS TS
(*) Extra European Market only
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5. OPERATING PRINCIPLE
The working area is ventilated by an unidirectional and recirculated air flow (1), whose rate is
about 70% of the total air volume, while the remaining 30% is exhausted (2) from the cabinet
via an output located on the top. The exhausted airflow draws an equal inward air flow volume
(3) through the front aperture that creates a barrier of protection for the operator. This air flow
barrier (contaminated from the outside) is suctioned (4) together with the laminar air flow
(contaminated by the product) to create 100% of the air flow (contaminated), then the cycle is
repeated.
The total air flow (100%) is supplied by a fan* blowing into a plenum from which a portion of
the air flow, after HEPA filtration, supplies the working area and the remaining, also after HEPA
filtration, is exhausted from the cabinet.
*NOTE: in the size 1.8 two fans are fitted.
HEPA filtration assures both the air sterility of the working space (product protection) and the
air sterility of the exhausted air (environment protection).
laminar air flow

1
(recirculated)
2 exhaust air flow
3 intake air flow
4 contaminated air
Pic. 4
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6. OPERATING INSTRUCTIONS
6.1 CABINET DESCRIPTION

LEGEND (see Pic. 5 and Pic. 6)
1. Main switch 13. Front aperture closure panel

2. Soft touch keypad 14. Exhaust air outlet
3. Run hours meter 15. Service socket
4. Mains inlet appliance with main fuses 16. DOP concentration reading port (100%)
5. Working surface 17. Panel for filters/fan compartment access
6. Spilt liquid sump 18. Side panel
7. Combustible gas solenoid valve 19. Motorblower
8. Inert, pressurised and gas fluids valve 20. Voltage free contact out (VFC)
9. Front window (manual actuation) 21. Alarm pilot light
10. Fluorescent lamps box 22. UV enabling microswitch
11. Mobile UV lamp socket 23. Alarm mute socket (use for service
12. Permanent UV lamp only)
24. Combustible gas valve
Pic. 5
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6.2 CONTROL PANEL
The control instruments are located on the

front of the right compartment, which forms
the control panel (Pic. 6).
The instruments located are the following:
Main switch (1)

Keypad (2)
Run hours meter (3)
Alarm pilot light (21)
References in the legend of Pic. 5
Pic. 6
6.2.1 Main switch
The main switch (Pic. 7) allows to switch ON/OFF the cabinet.

It breaks both live and neutral poles, and it allows the detection of
black out in order to report the event on display when the power is
reactivated. The key of the switch is removable in both ON and OFF
position as provided by standard EN 12469, annex H.3.4 (The Pic. 7
cabinet should only be turned on and off by means of a special
tool).
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6.2.2 Keypad
The running functions of the cabinet are activated by

the push buttons (keys) of the keypad and they are
visualised by relevant pilot LEDs (see Pic. 8).
The control keypad allows to the users the following

actions:
setting of the operating mode of the cabinet
(MODE 1/SAFE or MODE 2/stand by-UNSAFE);
switching ON/OFF fluorescent light;
switching ON/OFF service socket;
switching ON/OFF solenoid valve (combustible
gas);
switching ON/programming of UVC light cycle.
See Tab. 3 and Tab. 4 for meaning of keys and LEDs.
Pic. 8
Tab. 3 - Keys function of keypad

ENABLED
FUNCTION LED LED
KEY SYMBOL FUNCTION FUNCTION
DESCRIPTION COLOUR STATUS
MODE
LIGHT ON/OFF 1 and 2 BLUE FIXED
activation/ FIXED/
UV 2 YELLOW BLINKI
programming NG
SERVICE ON/OFF
1 and 2 BLUE FIXED
SOCKET
COMBUSTIBLE
ON/OFF 1 YELLOW FIXED
GAS VALVE
UV
PROGRAMMING
programme confirm 2 YELLOW OFF
FAN OFF switches to MODE 2 start up NA NA
ALARM RESET delete alarm notice 1 and 2 NA NA
NOTE: other functions are protected and accessible only by service and maintenance
personnel (see PART 2 of this manual).
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Tab. 4 – Visual alarms of keypad
FUNCTION ENABLED
LED LED
ALARM SYMBOL FUNCTION
DESCRIPTION COLOUR STATUS
MODE
A) airflow out of
range warning
open window
warning
1 RED FIXED
airflow too high

higher up red LEDs
airflow too low

lower down red LEDs
general warning BLINKING /

1
unsafe biological RED FIXED
condition 2 FIXED
NOTE 1: an additional alarm red LED is located above the keyboard.
6.2.3 Alarm pilot light
This warning light (Pic. 9) has redundant function of alarm, with

increased lateral visibility as slightly projecting from the casing.
Pic. 9
6.2.4 Run hours meter

The run hours meter (see Pic. 6 and Pic. 10) is activated when the
fan is running (MODE 1).
Its reading allows to monitor the life of the filter and to program
the routine maintenance.
The reset of hours is not possible, so it’s suggested to record the
date and the run hours at every replacement of the filter.
Pic. 10
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6.3 UTILITIES
6.3.1 Gas lines
WARNING! See ASSESSMENT OF RISKS, warnings 1.1.11 and 1.1.12
Inlet hoses for the combustible gas and for inert fluids and vacuum connections are
located on the rear of the right-hand compartment top (Pic. 11) and marked with
suitable labels (Pic. 13 and Pic. 14).
Pic. 11 Pic. 12
Pic. 13 Pic. 14
The taps are located on the right sidewall of the inside working chamber (Pic. 12).
The colour of the combustible gas tap knob is yellow.
The colour of the inert fluids and vacuum tap knob is black.
Combustible gas
Combustible gas line is controlled by a normal closed solenoid valve and a ball valve tap in
line, and it should be connected according to local regulations.
The solenoid valve is activated in MODE 1 only by the key of the keypad.
Alarm condition will deactivate the function.
Gas supply is automatically cut off by the solenoid valve in case of sudden lack of the vertical
airflow or in case of alarm activation (EN 12469, annex A.9). This is a safety measure to
prevent HEPA filter damage and possible risk of fire. Termination of alarm conditions and
automatic restart of the cabinet after black out will not reactivate the valve.
NOTE 1: the provision of gas supply is not recommended. Flames from gas burners interfere
with laminar airflow within the cabinet and may compromise containment.
NOTE 2: where practicable, disposable loops or use of electrical loop sterilizers are preferred
to gas burners.
Vacuum and inert fluids

Non combustible pressurised fluids line is equipped with a ball valve tap. Fluids must be non
aggressive towards materials of which the valve is made (body in brass, ball in chrome-
plated brass, gaskets in PTFE).
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6.3.2 Service socket
This socket is installed inside the working space, on the right side of the rear wall
(Pic. 12). On the 1.8 size there is a second socket on the left side.
The voltage supply is the same as the one of the cabinet.
Current load shall not exceed 3 A.
The relevant protection fuse is located in the right side compartment of the cabinet.
Always enabled, activated by the key of the keypad.
6.3.3 UVC light socket
WARNING! See ASSESSMENT OF RISKS, warnings from 1.1.6 to 1.1.8
This socket is installed inside the

working space, on the left side of
the rear wall (Pic. 15) and it is
exclusively dedicated to activate
EUROCLONE UV-C light box.
Enabled in MODE 2 only,

activated by the
key of the
keypad (see 6.3.7 and 6.7.6).
Pic. 15
6.3.4 Voltage-free contact (VFC) outlet (for service personnel only)
Service utility.
This is a voltage-free contact connector for remote monitoring

or control (Pic. 16), only for dedicated EUROCLONE
accessories.
It is placed on the compartment right-hand top of the cabinet,

to the left-hand position of the mains appliance inlet (Pic. 17).
Max voltage: 24 V, Max current: 1 A

Pic. 16
6.3.5 Alarm mute inlet (for service personnel only)
Service utility.
It is placed on the compartment right-hand top of the cabinet,

to the right-hand position of the mains appliance inlet (Pic. 17).
Needs dedicated service plug to mute the alarm.

Pic. 17
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6.3.6 Fluorescent lamp

The lighting of the working chamber is supplied by 2 fluorescent lamps. The lamps
are fitted inside the light box, below the access panel of the filter compartment, out
of the working area.
Always enabled, activated by the key of the keypad.

Interlocked with UV-C light.
6.3.7 UVC germicidal lamp
UVC lamp is fitted inside the working chamber (Pic. 18) on the back wall.
On request is available also an optional MOBILE KIT to connect to the dedicated
socket and to lay on the working surface (Pic. 19).
Both methods assure disinfection of the cabinet working area.
After the cleaning of working area surfaces was done, it is possible to sterilize them
by using UV-C (germicidal) radiations.
NOTE 1: not suitable in case of access to contaminated area or components (i.e. for
maintenance, replacement of filters or fan), before routine control and every time big
spillage of contaminated liquids occurs.
These cases require a complete and effective decontamination of the cabinet (see
8.2).
Generally the effectiveness of UV-C lamp disinfection (wavelength 253,7 nm)
depends on exposure time and on the nature of microorganisms: special tables
provide this information.
NOTE 2: ultraviolet (UV) radiation is not recommended for use in MSCs (see EN
12469, annex A.2).
Pic. 18 Pic. 19
Enabled in MODE 2 only, activated/programmed by the key of the

keypad (see 6.2.2).
Interlocked with fluorescent light.
By mean of suitable limit switches it is not allowed to light the lamp UV-C if the front
window or panel closing the front access is not fully closed.
The front glass window has a shielding capability higher than 98% of UV-C radiation.
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6.4 FRONT APERTURE
6.4.1 Glass window
The front window is made of laminated safety glass and it’s part of enclosure being a
protection against the UV-C radiations for the operator, determining moreover with
its position and integrity the inflow air barrier protection.
The breakage of the window compromises both the UVC radiations protection and the
air barrier containment.
The front window is tilting and equipped with gas springs in order to be easily
opened by hand, to ensure adequate support when is open and to guarantee a firmly
closed position.
During operating in MODE 1 (see 6.7.2), any position of the window except the
working position is UNSAFE and it will turn on the alarm devices.
During operating in MODE 2 (see 6.7.4), any position of the window except the
working position will disable the UV-C function.
6.4.2 Frontal closure panel
The closure panel (Pic. 20) allows you to completely close the front access of the
cabin. It is equipped with two key locks that lock the panel to prevent the opening by
unauthorized personnel.
Pic. 20
It can be used in the following conditions:
cabinet off (protection against dust and external contamination);
cabinet in MODE 2 (UV-C cycle or decontamination).
During operating in MODE 2 (see 6.7.4), the removal of the panel closing the front
access will disable the UV-C function.
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Two microswitches (Pic. 21 – pos. 1 and 2) enable the ignition of UV-C lamp and are
appropriately marked with a warning mark (attention).
Do not close the front access during operation in MODE 1.
During operation in MODE 1 (see 6.7.2), closing the front access will result in the
activation of the alarm.
Remove the panel before activating the MODE 1.
Pic. 21 Pic. 22
Placed the panel only in closed glass.
Once placed the panel, the cabinet appears as in Pic. 22.
Remove the keys from the panel and keep them in a safe place.
When not used, the panel can be hung on the

side of the cabinet (the default left side) using
the dedicated pin (Pic. 24) on which is hung on
the part arranged of the panel (Pic. 25).
The pin (Pic. 23) can also be

moved to the right side.
Pic. 23
Pic. 24 Pic. 25
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6.5 WORKING SURFACE

The working surface is flat and divided into sectors as per Tab. 5.
WARNING! Biological risk for the operator and the product.

• Wrong positioning of the shelf sectors may compromise the safety of the operator
and/or product.
• Pay attention to the insertion way (see Pic. 26).
Tab. 5 – Shelf sectors

SURFACE SECTORS
SHAPE CABINET CODE DESCRIPTION
VERSION Q.ty
LDA0000/LDA0300/ TOPSAFE 1.2/ BS/
solid 3
LDA0060 TOPSAFE 1.2 60220
LDB0000/LDB0300/ TOPSAFE 1.5/ BS/

solid 3
LDB0060 TOPSAFE 1.5 60220
LDC0000/LDC0300/ TOPSAFE 1.8/ BS/

solid 4
Pic. 26 LDC0060 TOPSAFE 1.8 60220
NOTE: width of the shelf sectors is different depending on the size of the cabinet.
6.5.1 Safe Working Area

The safe working area is the part of the working surfaces where the correct value and
distribution of the laminar flow guarantees the protection of the product against the cross
contamination and against the airborne contamination from outside entering the MSC through
the front aperture. Operate in the inner part of the safe working area (Pic. 27).
Details on the dimensions of the working area are available in relevant table (see 4.4).
Avoid that contaminated material passes over the sterile one when shifting it; keep
contaminated material separate from sterile one.
Keep sterile material far from the front edge of the working area and wastes in a back corner
of the working surface.
WARNING!
Risk for the operator.
• Obstruction of the intake

air grills may hamper
operator’s safety.
Pic. 27
• DO NOT obstruct the slots of the front and rear air intake grill.
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6.6 OPERATING DESCRIPTION

The cabinet may operate in two modes:
MODE 1 (SAFE operating):
it is the default mode (no action is required after turning on the cabinet) to start the
cabinet in SAFE operating condition;
MODE 2 (UNSAFE operating or STAND BY);
it is the second option of operating mode, for functions as cleaning, disinfection,
sterilisation or others. It can be set after turning on the cabinet only, during a short time.
This mode is not suitable to operate under biological risk.
Both conditions can be checked on the keypad (see 6.2.2).
NOTE 1: operating MODE is not switchable. The swap from MODE 1 to MODE 2 and vice-versa
needs restarting of the cabinet by the key main switch.
To change operating MODE restart the cabinet by main switch.
The key of the main switch should be always removed (both ON and OFF condition) and kept by
the operator in order to not allow actions by unauthorized persons.
To shut down the cabinet turn the main switch to OFF (O).
NOTE 2: to turn off the cabinet in case of emergency, in the absence of the suitable key,
remove the plug of the power cord from the mains socket (see 3.3.1).
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6.7 OPERATING PROCEDURE
6.7.1 CABINET STARTING

Turn the cabinet on by the key main switch (see Pic. 6 and Pic. 7).
The system starts showing on the keyboard the following steps:
AUTOTEST (blinking of LEDs and a series of beeps);
FIRMWARE REVISION (by mean of coded lighting of bar-graph LEDs);
COUNTDOWN of the LEDs bar-graph (LEDs turn off two by two from top to
bottom). During this step you can decide whether to continue in MODE 1
(SAFE) or to switch to MODE 2 (UNSAFE).
6.7.2 MODE 1 (SAFE)

NOTE: to run the MODE 1, first remove the frontal closure panel.
1. During COUNTDOWN (see 6.7.1), do not press any key and wait for completion of
WARM UP and safe operating condition.
During the warm up, the airflow is supplied at full rate for a short time and then is
decreased to reach the factory set value (SET POINT).
The SET POINT is visualized by the central green LED permanently lit, which will
flash as soon the airflow rate matches the set value.
The green central area of bar-graph LEDs represents the range of the allowed
values.
Airflow alarm will operate until inflow air value is in the correct range.
Small oscillations within green portion of bar-graph are normal.
2. Remove the key from the main switch and keep it in a safe place.
3. Leave the cabinet running for at least 10 minutes before starting the operation, in
order to stabilize of the airflow in the working space.
Now the cabinet is ready to operate.
6.7.3 MODE 1 ENABLED FUNCTIONS
In MODE 1 the following controls are enabled:
Solenoid valve for combustible gas line (see 6.3.1).

Service socket (see 6.3.2).
Cabinet lighting (see 6.3.6).
The active controls are the following:
Automatic control of the inflow air velocity.

Alarm devices activation.
Front window opening alarm.
Out of range airflows alarm.
Occurred blackout notice (visible warning).
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6.7.4 MODE 2 (UNSAFE or stand-by)
1. During the COUNTDOWN (see 6.7.1), press and hold the key for
4 seconds.
The system immediately switches to MODE 2. Ventilation is disabled.
Bar-graph LEDs are off. The audible alarm is disabled.
The general alarm LED is steady on to warn of the unsafe condition.
Light function is enabled.
2. Remove the key from the main switch and keep it in a safe place.
Now the cabinet is ready only for maintenance/decontamination.
6.7.5 MODE 2 ENABLED FUNCTIONS
In this condition the following controls are enabled:
Service socket (see 6.3.2).

Cabinet lighting (see 6.3.6).
UVC decontamination (see 6.3.3, 6.3.7 and 6.7.6).
The active controls are the following:
Unsafe MODE notice (visible warning).

Occurred blackout notice (visible warning).
NOTE 1: MODE 2 operation is indicated by permanent visual alarm (red LED).

NOTE 2: in MODE 2 audible alarm is disabled.
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6.7.6 UV-C ACTIVATION/PROGRAMMING
1. Turn on the cabinet and switch to MODE 2 (see 6.7.4).

2. Check the window be firmly closed.
3. Check the frontal access be closed (and locked with key) by the relevant panel.
4. Check the fluorescent lamps be turned off.
5. Press the key of the keyboard.

Using this key you set the time length of the UV-C cycle, indicated by the lower
area of the bar-graph red LEDs (see Pic. 28). Each press of the key turns on a
LED and increases the time of 10 minutes:
- 1 red LED lit = 10 minutes;
- 2 red LEDs lit = 20 minutes;
- and so on, up to 5 LEDs = 50 minutes.
At further touch of the key, all LEDs turn off, and then it’s possible to restart with
one LED, two LEDs, and so on.
6. Press the key of the keyboard to confirm.

After confirming there are 2 options:
- Wait for UV activation within 1 minute (skip to step 9); otherwise:
7. Press the key of the keyboard to programme the delayed

activation.
Using this key you set the delay time of UV-C cycle start, indicated by the central
area of the bar-graph green LEDs (see Pic. 28). Each press of the key turns on a
LED and increases the time of 1 hour:
- 1 green LED lighting up = 1 hour;
- 2 green LEDs lighting up = 2 hour;
- and so on, up to 11 LEDs = 11 hours.
At further touch of the key, all LEDs turn off, and then it’s possible to restart with
one LED, two LEDs, and so on.
Pic. 28
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8. Press the key of the keyboard to confirm the programme.
9. Programming is completed and the UV-C cycle will be activated with the delay and
the time set.
All keys are now disabled: il ciclo UV-C può essere fermato solo tramite
interruttore principale.
10. Remove the keys from the main switch, from the front panel and keep them in a
safe place.
Yellow LED of the key blinks, informing the user the cabinet is in
UV cycle waiting time.
The bar-graph (see Pic. 28) shows the setting:
- green LEDs show the time remaining to UV cycle activation (they will one by
one turn off every 1 hour, showing the remaining delay time);
- red LEDs at the bottom show the time length of the cycle.
Leaving the cabinet in this status, after the set time the UV cycle will be
activated.
During UV cycle, the red LEDs of the bar-graph lower area (see Pic. 28) they will
one by one turn off every 10 minutes showing the remaining activation time.
Turning off the cabinet by the main switch, the UV cycle programming will be
cancelled.
6.7.7 BLACK-OUT
In case of black-out, when the power is back the cabinet re-starts in the same mode
which was active when black-out occurred.
Safe ventilation conditions are automatically restored, as provided by Annex H.3.4 of
EN 12469 standard (Fan that stop due to a power cut should automatically be
restarted when the power is reactivated).
At reboot the unsafe visual alarm of the keypad will blink to warn
about event.
NOTE 1: the solenoid gas valve shall be always intentionally restored by means of
the relevant key.
NOTE 2: all the rest of utilities will be restored to the condition before the blackout.
NOTE 3: UV-C cycle will restart all over again.
NOTE 4: it’s up to operator evaluate the sterility of the product handled after this
event.
Press the key to cancel the BLACK-OUT notice.
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6.7.8 ALARMS
WARNING!
During alarm condition, safe conditions of the operator are NO longer ensured.
DO NOT operate when the alarm is on.
Visible alarms are located on the control panel (see 6.2).

Audible alarm is enabled only in MODE 1.
The following Tab. 6 shows how the alarms are activated.
Tab. 6 – Alarm events notice

EVENT MODE VISIBLE AUDIBLE
start up 1
airflow too low 1
airflow too high 1
opening of front window 1
occurred blackout 1 and 2
Layout of visible alarms is available in Tab. 4 and 6.2.3.

Muting is not permitted.
6.7.9 SWITCHING OFF
WARNING!
To turn off the cabinet in case of emergency, in the absence of the suitable key, remove
the plug of the power cord from the mains socket (see 3.3.1).
The means of disconnection of the cabinet from the mains supply is only the plug of the
mains supply cord.
To turn off the cabin under normal conditions, proceed as follows:
1. Turn off by the key main switch (see Pic. 6 and Pic. 7).
The cabinet immediately stops.
2. Remove the key from the main switch and.
3. Place the front closure panel locking it with the relevant keys.
4. Remove the keys from the front panel.
5. Keep the keys of main switch and front panel in a safe place
NOTE: this procedure applies to both the MODE 1 for the MODE 2.
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7. GENERAL RULES – GUIDELINES
In order to achieve better comfort for operators, EUROCLONE suggests the following
recommendations:
7.1 FEATURES OF LOCATION:

• Chair should assure fully support of the body;
• If the chair has adjustable low back support, match the chair backrest to support the
lower part of the spine;
• If different operators may use the cabinet, don’t assume the settings are properly set for
everybody;
• Vary the leg position frequently and avoid placing objects that limit the legroom under
the cabinet.
7.2 WORKING POSTURE:

• Keep forearms, wrists and hands aligned in a straight neutral position: avoid bending or
angling the wrist;
• Working intensely for long time poses risks: get up from the chair periodically and take
brief walks.
7.3 WORKING PROCEDURES:

• Operate the cabinet according to what prescribed by its definition (see 2.1, 2.2 and 2.3).
• DO NOT use the working area for storage of material.
• Request an efficiency test report of the cabinet after installation, filters and other
components replacement, any repositioning or dislocation, and in any case every six
months.
• Carry out the work according to a specific programme in which working steps, needed
materials and accessories (to be put in the cabinet) are listed.
• Put inside the cabinet only essential materials and equipment, before operating the
cabinet, so that nothing would pass through the front aperture during operation.
• Verify that there are no obstructions to the exhaust air outlet before turning the cabinet
on.
• Wait at least ten minutes of SAFE OPERATING condition (see 6.7) of the cabinet before
starting any operation, in order to allow the removal of the airborne contamination.
• Wash hands and arms always with a germicidal soap before and after work.
• Wear suitable clothing, long sleeves gowns with elastic cuffs, and rubber gloves.
• Put objects at least 15 cm off from the front window and operate in the innermost part of
the safe working area (see 6.5.1).
• Avoid that contaminated material passes over the sterile one when shifting it; keep
contaminated material separate from the sterile one, place the latter far from the
working area and put the wastes in a back corner of the working surface.
• Avoid obstructing the slots of the front intake grill with sleeves of the working garment;
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• DO NOT work when the fan is off (MODE 2), when audible or visible alarms are
activated, when the display does not work, when the front window is out of the working
position.
• Limit the activities inside the room where the cabinet is placed, because they could
disturb the air barrier containment and the laminar flow (LAF), with consequent decrease
in the operator and product protection.
NOTE: each time doors of the laboratory are open and closed, drafts are produced. It is
consequently advisable to put an advice board such as:
“Safety cabinet on – no entrance”.
• Keep the cabinet on for at least 10 minutes once the work is over, in order to remove
any contaminating substance from inside.
• Repeat the decontamination of inside surfaces once all objects as materials and
equipment have been taken out. For cleansing and decontamination, see par. 8.
• Pay special attention when removing cultures that have been accidentally poured inside
the working area: they could actually favour the growth of fungi and the spreading of
their spores with consequent contamination of the cabinet.
Page 45 of 82
80.021.029 r09 - July 2015
Part 1: User
8. CLEANSING AND DECONTAMINATION
8.1 CLEANSING
8.1.1 Painted surfaces

• Use common detergents, like soap solutions.
• DO NOT USE solvents or other paint aggressive products.
8.1.2 Stainless steel surfaces
• Specific products are recommended for normal cleaning.
• The disinfection of the inner surfaces of the working space shall be carried
out by using disinfectant liquids, following the relating instructions to prevent
misuse.
Decontaminate by swabbing with a disinfectant chlorine-free product.
WARNING! r
• Chlorine products damage stainless steel surfaces.
• DO NOT use products containing chlorine for stainless steel surfaces.
8.1.3 Front glass windows

• Disinfection of the glass window should be carried out with isopropyl alcohol.
WARNING! See ASSESSMENT OF RISKS, warnings 1.1.9 and 1.1.10
8.2 DECONTAMINATION
Routine decontamination of the working area surfaces can be carried out by means of UVC
radiation (see 6.3.7).
Otherwise, in several cases is required a complete and effective decontamination of the cabinet.
This type of decontamination of the cabinet is mandatory in the following cases:
before operations requiring access to any potentially contaminated area;

every time big spillage of contaminated liquids occurs;
before maintenance (fan and filters replacement);
before routine control;
before dislocation;
after any emergency.
Users shall identify the decontaminating method effective against the agent in question.
• A method of decontamination is described in the PART 2 – Service and maintenance.
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Class II Microbiological Safety Cabinets

GENERAL INFORMATION - FOREWORD

MAINS SUPPLY AT 230 V~ 50 Hz ONLY

MAINS SUPPLY AT 220 V~ 60 Hz ONLY

The manual consists of two parts:

• This manual refers to the above cabinet codes only.

WITHIN ITALY ABROAD

For any additional information, please contact the manufacturer:

Do not remove this label.

BIOLOGICAL HAZARD WARNING

UV-C HAZARD WARNING

MAIN GROUND TERMINAL BLOCK

Separated collection for electric and electronic components.

7. GENERAL RULES – GUIDELINES 44

PART 2: SERVICE AND MAINTENANCE 47

1.1.1 WARNING! General hazard. Risk category: 1

1.1.2 WARNING! Misuse hazard. Risk category: 1

1.1.3 WARNING! Biological hazard. Risk category: 1

• DO NOT work when alarm is on.

1.1.4 WARNING! Biological hazard. Risk category: 1

1.1.5 WARNING! Biological hazard. Risk category: 1

1.1.6 WARNING! Biological hazard. Risk category: 1

1.1.7 WARNING! UVC radiations hazard. Risk category: 1

• DO NOT allow unauthorized people or general public to approach the cabinet.

1.1.8 WARNING! UVC radiations hazard. Risk category: 1

• DO NOT allow unauthorized people or general public to approach the cabinet..

1.1.9 WARNING! Pinch hazard. Risk category: 1

1.1.10 WARNING! Electric shock hazard. Risk category: 1

• Adequate information for user.

• This operation must be performed by adequately trained and authorized Service

1.1.12 WARNING! Liberated gases and explosion hazard. Risk category: 1

• Use Bunsen burner only with flame control or piezoelectric ignition.

1.1.13 WARNING! Liberated gases and explosion hazard. Risk category: 2

• Decontamination shall be carried out solely by specialized and authorized

2. RULING, STANDARDS AND DEFINITION

2.1 EC DECLARATION OF CONFORMITY

HEREWITH DECLARES THAT THE FOLLOWING PRODUCTS:

DESCRIPTION: CLASS II MICROBIOLOGICAL SAFETY CABINETS

COMPLY WITH THE PROVISIONS OF THE FOLLOWING DIRECTIVES:

2006/42/EC Machinery Directive

AND THAT THE FOLLOWING STANDARDS HAVE BEEN APPLIED:

EN 61010-1:2010 Safety requirements for electrical equipment for

EN 61326-1:2013 Electrical equipment for measurement, control and

EN 12469:2000 Biotechnology - Performance criteria for microbiological safety

2.2 DECLARATION OF CONFORMITY FOR EXTRA UE MARKETS ONLY

CODES: LDA0060; LDB0060; LDC0060

IEC 61010-1:2010 Safety requirements for electrical equipment for

2.3 CABINET DEFINITION

TOPSAFE cabinets are defined as follows:

• MSCs ducted outside the laboratory require an additional validation by qualified

WARNING! See ASSESSMENT OF RISKS, warning 1.1.2

WARNING! See ASSESSMENT OF RISKS, warning 1.1.1

3.1 CHOICE OF LOCATION

WARNING! Biological hazard.

3.2 FLOOR STAND

WARNING! Instability hazard.

• This operation should be carried out only by qualified service personnel.

3.3 CONNECTING TO THE MAINS

3.3.1 Mains socket

• Connect the cabinet to a dedicated power line, with a near-based grounding