Republic of the Philippines

Department of Education
REGION XII
DIVISION OF SULTAN KUDARAT
BAMBAD NATIONAL HIGH SCHOOL
Isulan, Sultan Kudarat, 9805

ASSESSMENT OF SYNERGISTIC EFFECTIVENESS OF

AVOCADO OIL, OLIVE OIL, AND ALOE VERA GEL AS AN
ORGANIC MOISTURIZER

A Research Paper Presented to the

Senior High School Department of Bambad National High School,
Bambad, Isulan, Sultan Kudarat

In partial fulfillment of the requirements

in Practical Research and 3I for the S.Y. 2024-2025

Proponents
Cachuela, Cyan Aira H.
Diosana, Ryan N.
Launio, Angel G.
Margallo, Nicolette Cookeii A.
Peroy, Christian B.
Segura, Rowel P.

January 2025
Chapter 1
 The Problem and Its Setting

Introduction
Moisturizers are essential for maintaining skin health,
especially in dry or harsh climates, and can help improve skin
texture, elasticity, and overall appearance. Moisturizers also serve
as vehicles to carry nutrients to the skin. They give practical skin
benefits like making the skin smooth and silky, enhancing skin
moisture, and improving skin optical qualities. Vitamins, natural
anti-oxidants, peptides, skin-lightening agents, natural anti-
inflammatories, and exfoliants are a few examples of these
substances. (Mawazi et al., 2022).
Organic moisturizers involve a multifaceted exploration into
the skincare industry’s shift towards natural and sustainable
products. Organic personal care products market has a higher
potential to grow in the Asian market when compared to the non-
organic personal care sector (Wiwatanapusit, 2017). It emerges
against the backdrop of increasing awareness about the potential
harmful effects of synthetic chemicals in traditional skincare
formulations.
The increasing awareness of the harmful effects caused by
synthetic ingredients found in skincare products has led to an
extensive search for natural bioactive. On the other hand, compared
to synthetic skincare and cosmetic ingredients, herbal products are
mild and biodegradable, exhibiting low toxicity..
In today’s generation the use of Organic Moisturizers is
exponentially increasing due to the public awareness of the value of
self-care and wellbeing due to the pandemic. According to (Bize,
2021).
Despite the widespread use and popularity of organic
moisturizers, there is a lack of comprehensive research assessing
their long-term effectiveness in comparison to synthetic
moisturizers. This research gap highlights the need to investigate

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the specific benefits and drawbacks of organic moisturizers,
including their impact on different skin types and conditions, to
provide evidence-based recommendations for skincare routines.
The primary purpose of assessing the effectiveness of organic
moisturizers lies in understanding their ability to provide hydration
and nourishment to the skin using natural ingredients. Organic
moisturizers are formulated without synthetic chemicals, which
appeals to individuals seeking skincare products that are gentle,
sustainable, and potentially less irritating to sensitive skin types.
The Importance study is to aids consumers in making informed
decisions about skincare products, aligning with their preferences
for organic, eco-friendly options. Also, it contributes to the growing
demand for sustainable beauty practices, encouraging innovation
and research in natural skincare solutions. And, understanding the
efficacy of organic moisturizers can benefit dermatologists and
skincare professionals in recommending suitable products for
different skin concerns, promoting overall skin health and well-
being.

In the realm of organic skincare, the demand for natural

moisturizers has significantly increased, with a focus on ingredients
that offer both efficacy and safety. Avocado oil, olive oil, and Aloe
Vera gel are widely known for their individual moisturizing, anti-
inflammatory, and healing properties. However, despite their
individual benefits, limited research has been conducted on the
synergistic effects of these ingredients when combined as a
moisturizer. The primary problem addressed in this study is the lack
of scientific evaluation regarding the combined effectiveness of
avocado oil, olive oil, and Aloe Vera gel as an organic moisturizer.
While each ingredient individually has demonstrated potential in
improving skin hydration and elasticity, it remains unclear whether
their combined use enhances or reduces their moisturizing
properties. Additionally, there is a need to determine if the

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synergistic application of these three substances can provide
superior moisturizing results compared to the individual
components.

Statement of Problem
Generally, this study aims to quantitatively assess the
synergistic effectiveness of Avocado Oil, Olive Oil, and Aloe Vera Gel
when used together as an organic moisturizer. This study will be
conducted in January-April 2025 during the school year 2024-2025.

Specifically, it will examine the following research questions:

1. What is the combined moisturizing effectiveness of
Avocado Oil, Olive Oil, and Aloe Vera Gel.
2. Does the combination of these ingredients result in
superior skin hydration, as measured by the following
assessment methods,
a. Corneometry,
b. Transepidermal Water Loss,
c. Visual/Tactile Assessment, and
d. Skin Patch Test?
3. Is there a significant difference in the sensory
characteristics of Avocado oil, Olive oil, And aloe vera
moisturizer and Commercial Moisturizer in terms of Scent,
Texture and Lightness?

Hypothesis
Ho: There are no significant difference in the sensory
evaluation of synergy of Avocado Oil, Olive Oil, and Aloe Vera
Gel moisturizer compared to commercial moisturizer.

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Significance of the Study
This study aims to determine the synergistic effectiveness of
Avocado Oil, Olive Oil, and Aloe Vera Gel as organic moisturizer the
different parameters. Thus, the findings of this study will be
beneficial to the community, consumer, respondents, researchers,
and to the future researchers.

To the Community:Assessment of skin hydration brings a huge

contribution to the health and well-being of the community. The
study highlights a behavorial change among the community, calling
for the people to make healthier skincare choices. This would take
persons to cater for healthier skincare choices, hence making an
informed and conscious decision towards personal care. This is
bound to enhance skin conditions, increase people's self-confidence,
and uplift the community's well-being level to a higher degree.

To the Consumer: The study will present a compelling case for the
organic moisturizer industry and thus lead consumers to favor
healthier choices. This switch may enable consumers to use natural
and sustainable options in their skincare routine, leading to less
exposure to harmful chemicals and a more environment-friendly
approach towards beauty.

To the Respondents: The respondents gain from the study in new

facets of insightful knowledge concerning the efficacy of organic
moisturizers. With that knowledge, they can make informed product
choices that will most likely improve their skin condition. Because
during this research the respondents experienced agency and
ownership over their decisions about skincare.

To the Researcher: The study gave the researchers a chance to

enlarge their frontier of consideration with regard to the efficacy of
organic moisturizers on skin moisturizing. It may bring forth further

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studying of the special properties of organic ingredients as
concerned with skin health, one targeted towards formulating other
and more efficient and sustainable skincare options.

To Future Researchers: This paper would stand as a good source, or

base, for subsequent researchers into skincare and organic
materials. It thus presents an opportunity for more exploration of
research based on its findings concerning comparatives and filling
gaps of knowledge that this study cannot find. It will hence enable
other researchers to validate it further.

Scope and Delimitations

This study focuses on assessing the effectiveness of an
organic moisturizer formulated with Avocado Oil, Olive Oil, and Aloe
Vera Gel as primary ingredients. The research will be conducted
within a specified timeframe, from January to March 2025, at
Bambad National High School. It aims to evaluate various properties
and effects of this organic moisturizer, including its appearance,
scent, texture, spreadability and lightness.
The study will be limited to respondents within Bambad
National High School, specifically focusing on students and
individuals in the community during the study period. The
moisturizer formulation is restricted to avocado oil, olive oil, and
aloe vera, excluding other possible natural or synthetic ingredients
that may be used in commercial and organic moisturizers. The study
will take place over a month period, limiting observations to short-
term effects rather than long.

Definition of Terms
For clarity and a better understanding of the concepts, the
following terminology used in the study is defined both conceptually
and operationally.

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Avocado Oil. Conceptually, one of the primary natural ingredients
used in the organic moisturizer evaluated in this study. Known for its
richness in vitamins and fatty acids, avocado oil provides deep
nourishment and hydration, making it beneficial for both dry and
aging skin.

Olive Oil. Conceptually, serves as another key ingredient in the

organic moisturizer. It is valued for its high antioxidant content,
which helps to moisturize and protect the skin against damage. The
study investigates how olive oil contributes to skin smoothness and
resilience.

Aloe Vera. Conceptually, it is a soothing, hydrating plant extract

incorporated in the organic moisturizer being studied. Its anti-
inflammatory and healing properties make it suitable for sensitive or
acne-prone skin, helping to calm irritation while providing hydration.

Viscosity. Conceptually, refers to the thickness and consistency of

the moisturizer in this study, impacting how easily it spreads over
the skin. The study evaluates viscosity to determine if the
combination of avocado oil, olive oil, and aloe vera results in an
ideal texture that enhances the application experience.

Skin Barrier Function. Conceptually, it refers to the outermost layer

of the skin, which protects against environmental pollutants and
retains moisture. This study focuses on how the organic moisturizer
supports the skin barrier, enhancing hydration and resilience.

Dry Skin. Conceptually, it refers to skin that lacks adequate

moisture, resulting in a rough or flaky texture. This research
assesses the effectiveness of the organic moisturizer in combating
dryness and restoring moisture to dry skin types.

Synergistic Effectiveness. Conceptually, it refers to the combined

power of avocado oil, olive oil, and aloe vera in the organic

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moisturizer, potentially leading to greater benefits than if each
ingredient were used alone.

Eco-Friendly. Conceptually, relates to the use of organic and

sustainably sourced ingredients that are gentle on the
environment. The focus on eco-friendly products aligns with
the increasing demand for sustainable skincare solutions and
is part of the study’s goal to evaluate consumer satisfaction
with natural skincare.

Framewok of the study

This part includes the theoretical framework which presents
the theory and concepts as the basis or foundation of the study and
the conceptual framework which shows the relationship among
variables used in the studies.

Theoretical Framework
This theoretical foundation of this study is Synergistic Effect
Theory, which has been extensively used in industries like skincare,
cosmetics, and medicine. As stated by (Wang and Weller, 2006).
According to the notion, combining many bioactive substances can
increase their biological activity and possibly improve their efficacy
compared to using them alone. This strategy is especially pertinent
to skincare, as mixing natural products can maximize their
advantages and reduce the possibility of discomfort from excessive
concentrations of individual chemicals.
The Synergistic Effect Theory serves as the basis for the
hypothesis in this study that the combination of avocado oil, olive
oil, and aloe vera may produce more effective moisturizing benefits
than either ingredient alone, providing a comprehensive and potent
skincare treatment.

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 Conceptual Framework

Independent Variable Dependent Variable

Skin Hydration

a.Moisturing Effeciency

b.Absorption Rate

c.Skin Sensitivity

d.Long Term Effects

Avocado oil, Olive oil, and Sensory Characteristics

Aloevera Gel Moisturizer
a.Appearance

b.Scent

c.Texture

d.Spreadability

e.Lightness

Figure 1. Schematic diagram showing the relationship of the

variables.

The schematic diagram in Figure 1 presents the independent

and dependent variables used in this study. Avocado oil, Olive oil
and Aloevera Gel is identified as the independent variable because
it is the factor that the researchers are manipulating to observe its
impact on the effectiveness of skin treatment. On the other hand,
the Moisturizing Efficiency, Absorption rate, Skin Sensitivity, And
Long-term Effectiveness is the dependent variable because it is the
outcome that depends on the independent variable (the organic
moisturizer used). In this study, the effectiveness is measured
through specific indicators, including Moisturizing Efficiency,
Absorption rate, Skin Sensitivity and Long-Term Effectiveness.

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 Chapter 2
Review of Related Literature and Studies

This chapter contains a review of related literature and related

studies after the in-depth search done by the researchers which has
bearing on the study. This also presents the conceptual literature
and studies that are significantly relevant to the study. The review
of literature is thematically organized based on the variables used in
the study.

Avocado Oil
Avocado (Persea americana Mill.) is a fruit native to Central
America, grown in warm temperate and subtropical climates
throughout the world. The pulp of this fruit contains about 60% oil,
7% skin, and approximately 2% seed. Cosmetic application of
avocado oil involves its use in shampoos for babies and for
damaged hairs, skin and body care, nourishing creams for damaged,
dry and sensitive skins, suitable for baby skin, effective in sun
products because of its protective action and it could also be found
useful in massage oils and products for around the eyes. Because of
its richness in vitamin A, E and D in un-saponifiable compound, the
it contains lecithin an essential ingredient for the skin,hence
avocado oil has hydrating and regenerating properties and therefore
useful as an anti-ageing and anti-wrinkle products, repairs dry skin
and renders elasticity to the skin (Ordu, 2018).

Organic Moisturizer
In ancient times, Greeks utilizes olive oil to protect their skin
from the sun. Over the years, South Americans have historically
turned to extracts from rice jasmine and lupine plants as part of
their skincare regimen for sun protection. Sunscreen, often referred

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to as sunblock, is a gel-like moisturizer designed to absorb, reflect,
or scatter UV radiation, thus preventing sunburn. The first known
sunscreen moisturizer was developed by Eugene Sculler in France in
1935, It was later introduced to the commercial market by Franz
Greiter in 1946. Since then, sunscreens have become a crucial
skincare product globally. In 1990, the United States spent $525
million on sun protection products, with annual increases to combat
the damaging effects of UV radiation on the skin (Obanla et al.,
2019).
The demand of organic and natural products is increasing
nowadays. Coconut oil, which is known for its advantageous fatty
acids, has primarily served as a key ingredient in oil-based and
moisturizing products. There is growing interest in exploring the
enzymatic synthesis of liquid wax esters derived from coconut oil
and unsaturated fatty alcohols as a potential alternative natural
ingredient in wide range of industry (Ungcharoenwiwat & H-Kittikun,
2020).
Nothing beats the power of natural substances when it comes
to skincare. The kitchen has always played a vital part in skin care,
from our grandmothers to our moms. Beetroot is a magical cooking
ingredient with incredible health advantages. Beetroot has Vitamin
C, which is a great element that makes your skin brighter and more
even in tone. Beetroot on lips helps to lighten and brighten your lips
by removing dark spots. Beetroot's pink tint also provides a tinge of
pink to your lips. Beetroot is really beneficial to your lips. Beetroot
treats dry and chapped lips and is the greatest natural lip
moisturizer. Beetroot makes your lips juicy and full. Beetroot juice
gives your lips an instant glow and makes them appear more
moisturized, nourished, and juicier (Putri et al., 2022).

Moisturizer
Ultraviolet has harmful effect on our skin which further led to
development of inorganic and synthetic organic UV filters that can

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reduce the harmful effect of exposure to sunlight. Moisturizers are
extensively used as a self-care product to improve the ability of the
skin. Whilst UV filters and moisturizers have a good quality, they
may prevent the effects of contact sensitivity, oestrogenicity and
even tumorigenic effects on human skin. The accumulation of these
chemical could be harmful, there is a safer alternative way
especially photosynthetic organisms like cyanobacteria which have
developed mechanisms for coping with high UV irradiation and
desiccation. These microorganisms produce UV screening
compounds such as mycosporine-like amino acids and scytonemin,
which are good candidates as alternatives to current synthetic UV
filters (Derikvand, 2017).
Moisturizers design to maintain water content of the skin
between 10% and 30%. The water is essential to our skin to
maintain hydration, improve the barrier, and prevent dryness. Skin
that are lack of water and rough to touch, water is necessary to
allow skin distensibility. Skin hydration is important to maintain
intact skin barrier, tactilely perceived as smooth, soft skin (Draelos,
2018).
Conventional moisturizers establish a surface-level occlusive
barrier on the skin, whereas natural lipids like ceramides penetrate
the stratum corneum (SC). These lipids are synthesized within the
keratinocytes, undergo processing in lamellar bodies, and are
subsequently secreted back into the stratum corneum to become a
part of the skin's dermal matrix (Spada et al., 2018).
Moisturizers have several benefits aside from skin hydration.
These encompass anti-inflammatory properties, where specific
components within moisturizers, such as glycyrrhetinic acid,
palmitoyl-ethanolamine, telmesteine, Vitis vinifera, ceramide-
dominant barrier repair lipids, and filaggrin breakdown products,
display significant anti-inflammatory effects. These mechanisms
include the inhibition of cyclooxygenase activity, regulation of
cytokines, and reduction in proinflammatory prostanoid production,

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resulting in soothing relief for inflamed skin conditions like
dermatitis. Moisturizers also offer antipruritic benefits, especially
water-based variants that create a cooling effect through skin
surface water evaporation. Additionally, certain moisturizers may
contain menthol, which imparts a cooling sensation that effectively
relieves itching and related symptoms. In terms of antimitotic
effects, some moisturizers containing mineral oils possess mild
epidermal antimitotic properties, making them useful for individuals
with skin conditions characterized by increased epidermal mitotic
activity, such as psoriasis. By moderating cell proliferation in the
outer skin layer, these moisturizers can help manage and alleviate
symptoms associated with such conditions. Furthermore,
moisturizers containing hyaluronic acid, a common ingredient, have
been demonstrated to accelerate wound healing. This property
holds significant promise in skincare and dermatology, as it
enhances the body's natural ability to repair damaged skin,
resulting in faster and more effective wound healing (Purnamawati
et al., 2017).

Dry Skin
Dry skin is a common and complex issue that is often
associated with irritation, itching, discomfort, and even visible skin
problems. It greatly affects the adults broadly and requires specific
care. Recognizing established risk factors can aid in pinpointing
individuals who may be at risk of worsening symptoms (Agustine,
2018).
Dry skin is the most prevalent clinical dermatologist issue,
which leads to discomfort and is often associated with itching,
redness, and flaking. These physical discomforts can also have
psychological implications. The skin, being the body's largest organ,
serves as a vital protective boundary between us and the outside
world. The primary role in this barrier function is attributed to the
epidermis, particularly the stratum corneum (SC). Dry skin,

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characterized by itching, redness, and scaliness, is often linked to
disruptions in enzymatic processes, partly due to reduced water
content in the SC. The hydration of the stratum corneum largely
depends on the composition and quantity of intercellular lipids,
which control water loss, and the presence of hygroscopic
substances known as natural moisturizing factors, responsible for
retaining water in the stratum corneum. The skin's primary
functions include preventing water loss and shielding the body from
harmful substances and microorganisms. Skin barrier impairments
are common in various skin conditions, but the effects of aging on
skin barrier function remain a topic of debate, with conflicting
results have been reported (Jurakic, 2018).

Consumers Preferences in Buying an Organic Product

Women in their 20s and 40s with income above 500 dollars,
and educated are more likely to buy organic skincare products.
However, most people still purchase conventional skincare products
mainly because they are a lot cheaper compared to organic
products. Consumers also lack information about the differences
between organic and conventional skincare products. Many believes
that the two categories are equally effective and beneficial to
health. Additionally, people don't always take care of their health,
and they rarely read the ingredients list on skincare products.
consumers’ tendency to buy more certified organic skin care with
eco-friendly packaging increases along with an increase in income
and not according to their educational attainment. Consumers
needs to be better educated about the benefits of organic skincare
products and the importance of health and the environment when
making decisions about skincare products (Drăgan & Petrescu,
2013).

Aloe Vera

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 natural ingredients that have benefits as skin moisturizers,
one of which is aloe vera (Aloe vera L.). Aloe vera contains vitamins
A, B1, B2, B3, B12, C, E, choline, inositol, as well as folic acid,
magnesium (Mg), calcium (Ca), chromium (Cr), potassium (K),
sodium (Na), iron (Fe), and zinc (Zn). Currently, aloe vera is widely
cultivated in Indonesia because it is a natural ingredient used in the
production of cosmetics, food, beverages, and medicines (Imani &
Shoviantari, 2022; Mardiana & Ambarwati, 2021).In this case,
sufficient water intake can provide nutrients to skin cells and
improve skin elasticity, making the skin healthy, supple, and well-
moisturized. One solution for dry skin is the use of moisturizers in
gel form. Gel is a topical preparation that is easy to apply to the skin
because it contains water that can cool, soothe, moisturize, and
easily penetrate the skin ( Ramdhini, 2024).

Olive oils

Olive oil is abundant in natural emollients and vital fatty acids

that hydrate and moisturize the skin. They shield the surface, stop
moisture loss, and maintain suppleness of, the skin. They are rich in
vitamins, nutrients, and antioxidants that nourish the skin. Olive oil
has antiinflammatory, moisturizing, and antiaging properties
because it contains antioxidants and healthy fatty acid. 0 Olive oil
has been widely used in several cosmetic products, such as skin and
hair care formulations. There are several studies reporting the
potent antioxidant activity of olive oil. The active compounds
responsible for the antioxidant activity, belong to three different
classes, including simple phenols, secoiridoids, and lignans.
Moreover, olive oil may be used to protect the skin from ultraviolet
B in the sunlight based on a study reporting that mice receiving
olive oil after UVB exposure showed a significantly lower number of
developing tumors per mouse than those in the control group
receiving nothing (Nunes, 2021). Therefore, the aims of the present
study were to develop MEs from olive oil and characterize their

15
antioxidant activity and skin moisturizing properties for further
applications in cosmetics. Olive oil and its derivatives are
extensively used in the preparation of many pharmaceutical
formulations for therapeutic or cosmetic applications. Olive oil is
known to be beneficial in the treatment of psoriasis by increasing
hydration of the stratum corneum and through the keratolytic effect.
Olive oil is currently used in topical applications for the treatment of
several skin conditions, including dry skin, itching, and inflammation
as well as disorders such as psoriasis and atopic dermatitis.

Chapter 3
Research Methodology

This chapter includes all the procedures of the study and the
details of the progressive development of the research to be
conducted. It comprises the research design, the locale of the study,
participants and respondents of the study, data gathering
procedures, analysis of data, and research ethics.

Research Design

Complete Block Design (RCBD) since the skin reacts

differently to similar moisturizing treatments. The overall goal of this
experiment was aimed at determining how well avocado oil, olive
oil, and aloe vera gel work together as a natural moisturizer. This
was done using alternative methods for testing results:
Corneometry, TEWL (Transepidermal Water Loss), Visual/Tactile
Assessment, And Skin Patch Tests. As skin reactions may be
involved, it is somewhat complex and upon individual, so RCBD was
adopted to make sure that any change in the skin could be

16
 attributed to the treatments and anything else might be happening
outside of the experiments.

Descriptive statistics will then be calculated, including means

and standard deviations, to summarize the key data points such as
skin hydration levels, TEWL rates, and sensory evaluation scores
related to lightness, texture, and scent. A T-test will be conducted to
assess whether there is a significant difference between the
effectiveness of an organic moisturizer composed of avocado oil,
olive oil, and aloe vera gel and a commercial moisturizer.

Gathering Data

Preparation of Materials

Extracting of Avocado, Olive and

Aloe vera

Formulating the Organic

Moisturizer Assessment Methods
-Corneometry
-Transpideral Water Loss
Testing of Moisturizer
-Tactile/Visual
Assessment

Sensory Evaluation -Skin Patch Test

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 Treatments Parameters

- Synergy of -Appearance
Data Analysis
Avocado oil,
Olive oil, and -Scent
Aloevera Gel
-Texture
Moisturizer

-Commercial -Spreadability
moisturizer
-Lightness

Figure 1. Overall Framework of the experiment.

Research Locale
This study will be conducted in Isulan, Sultan Kudarat,
specifically at Bambad National High School. Situated 200 meters
from the national highway and 5.2 kilometers from downtown
Isulan, this location was chosen fosr its convenience and
accessibility. Bambad National High School, also fondly referred to
as the “Paradise of Learning,” and it is renowned for the numerous
achievements and active involvement of both its students and
teachers.

The particular site for the research within the school was the
science laboratory. This space was chosen due to its large size and
capacity to accommodate various experiments. Furthermore, the
laboratory was well-equipped with the necessary tools and
apparatus, ensuring an efficient and effective execution of the study

The Testing of Cornometry, Transpideral Loss, Tactile and

Visual assessment and Skin patch test of Organic Moisturizer
including will be conducted at Notre Dame of Marbel University

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 (NDMU), located at Rafael Alunan Avenue, Barangay Zone III,
Koronadal City, South Cotabato. The university spans around 8
hectares and is situated 1,106 meters above sea level,
approximately 4.04 kilometers from the Koronadal City Hall.

Notre Dame of Marbel University is a reputable Catholic

institution based in South Cotabato, marked by excellence in
academic pursuit. It has an enrollment of over 4,000 students
who are handled by more than 300 qualified staff members of the
faculty. NDMU is recognized for its commitment to providing high-
quality education. The research will take place at the science
laboratory of the university using in vitro conditions with a
spacious facility and well-equipped with all the necessary tools
and equipment for the experiments needed for this study.

Figure 2. Geographical Map of the Study Locale

Respondents of the Study

A purposive sampling will be used whereby individuals will be
selected based on their willingness to participate in the study while
at the same time qualified , especially upon having normal skin or
minor dermatoses but having dryness or irritation. This would
ensure that the subjects have a baseline condition appropriate
enough for assessing the effectiveness of the moisturizer. Thirty
(30) purposely selected students who are available during the
sensory evaluation from Bambad National High School who agree to

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participate in the study and use the organic moisturizer are the
sample of this study.

Data Gathering
1.Preparation of Materials
All necessary tools and equipment will be readily accessible at
Diosana’s residence in Purok 4, Kudanding, Isulan, Sultan, Kudarat.
Essential items, including containers, jars, knives, scissors, fine
mesh strainers, cheesecloth, and blenders, will be meticulously
cleaned and sanitized before use. Following utilization, each item
will be thoroughly cleaned again and returned to its designated
area.

2.Extracting of Avocado, Olive and Aloe vera

The avocado will be purchased from the neighbourhood
market around the area. of Sultan Kudarat. To remove impurities
like dirt, grit, and dust, the avocado will be washed thoroughly in
running water. After washing it, the fruit will be cut into halves using
a knife, and the flesh is scooped out using a spoon. The scooped
flesh will be placed in a bowl and was mashed with a fork until it
becomes pure. Avocado paste will be spread evenly onto a baking
tray and bake in a preheated oven. The paste will dry and become
slightly brown. Once the avocado paste is fully dried, place a filter
material like cheesecloth will be placed over the container, the dried
paste will be added to the filter, and will be slowly applied the
pressure to allow the oil to drip into the container. After pressing,
the oil will be transferred to sealed dark glass bottles and stored it
in a cool, dark place.
The Olive will be purchased from the neighbourhood market
around the area of Sultan Kudarat. To remove impurities like dirt,
grit, and dust, the olive will be washed thoroughly in running water.

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After washing, the olive will be crushed using a blender. A colander
or sieve will be lined with cheesecloth, and the olive paste will be
spooned onto it. The ends of the cloth will be gathered and tied it
into a bundle. A heavy object will be placed on top, and the mixture
will be left drain for 30 minutes. The mixture will be stand covered
for 12-24 hours or until the water and oil separate. The olive oil will
be skimed from the top and collected in a bottle.
The Aloe Vera will be collected around Diosana’s residence,
Purok 4, Kudanding, Isulan, Sultan Kudarat. Fresh aloe vera leaves
will be chosen for being healthy and have not been damaged. It
should be thick and plump and have a bright green color. The
collected leaves will be cleaned by rinsing them thoroughly with
water to remove any dirt or debris. After cleaning, the outer layer of
the leaves will be carefully removed using a sharp knife to reveal
the gel-like substance inside. The gel will be scooped out using a
spoon and placed in a clean, sterile container. To purify the gel, the
researchers strained it through cheesecloth to remove any
impurities, and the purified Aloe Vera extract will be stored in a
clean container.

3.Formulating The Organic Moisturizer

The ingredients are commonly found in the market. The

researchers will ensures that all materials used in the preparation
are organic and of the highest quality to ensure accurate and
reliable results. The necessary ingredients that will be gathered,
include Avocado oil, Olive oil, and aloe vera gel. The aloe vera gel
will be extracted from the aloe vera plant, and the Avocado and
Olive oil will be extracted and filtered. The moisturizer was made
from a combination of avocado oil, olive oil,and aloe vera gel
emulsifying wax and beeswax, This will be melted and mixed
together.

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4.Testing of Moisturizer
Corneometry: The technique is non-invasive and estimates
the hydration levels of the skin by determining its capacitance
in the outermost layers. The more water present in the skin,
the greater the capacitance. Values will be taken both before
and after some treatment has been applied. A Corneometer
(or a similar device like the Corneometer CM 825) will be used
to measure the dielectric constant of the skin, which directly
correlates to its water content.

Transepidermal Water Loss (TEWL): TEWL is a measure of the

rate at which water evaporates from the skin's surface. Lower
TEWL values correlate to an improved skin barrier, indicating
better hydration and possibly a better uptake of the
ingredients of the applied treatment. Pre-treatment and post-
treatment evaluations will be conducted against the changes
in skin barrier function.The measurement will be done by
using a Tewameter evaporimeter, Dermalab TEWL module, or
Servo Med evaporimeter.

Visual and Tactile Assessments:These subjective methods

involve visual observation and tactile assessment of the skin
to evaluate skin sensitivity and overall appearance. Trained
personnel will observe the skin for signs of irritation (redness,
dryness, etc.) and assess the skin's texture. This will be done
before and after treatment application, observation shall be
through naked eye and by touching. A standard scoring
system or checklist can be used to evaluate the respondent,
which can minimize the bias during assessment.

Patch Tests on Skin: The final testing period checks the long-
term safety of the moisturizer with a set of participants' skin.
A small amount of each treatment would be applied to a

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 specific area of the person's skin. The skin would then be
observed for reactions, such as irritation, redness, allergies,
which would take place over 12-24 hours. This helps check
adverse reactions and long-term compatibility. Standardized
allergens or the product itself will be placed in small chambers
mounted on tape to ensure controlled application. The
observation will be done visually.

5. Sensory Evaluation
This study used sensory evaluation of the organic lip balm
using criterion variables to the fifty-five (30) respondents of the
study in order to determine the synergistic effectiveness of avocado
oil, olive oil and aloe vera as an organic moisturizer. The responses
of the respondents are enumerated, examined, and interpreted.
Participants will be asked to evaluate the moisturizer in terms
of its lightness, texture and scent based on their sensory
characteristics. Before testing the given samples, the respondents
undergo skin testing to ensure their safety and avoid any allergic
reactions during the evaluation. The data collected from the sensory
evaluation was recorded and analyzed to determine the overall
effectiveness of organic moisturizer.
Basing on a five-point scale, a modified Sensory Evaluation
Tool (S.E.T.) was utilized to retrieve data. Organic Moisturizer will
evaluated with the following scores on their description: 5- like
extremely, 4- liked, 3- neither like nor dislike, 2- dislike, 1- dislike
extremely. These scores was assigned for the evaluation of
lightness, texture and scent, and effectiveness of organic
moisturizer.

Data Analysis

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Table 1. Scoring Rubrics evaluating Avocado, Olive, and Aloe vera
Organic Moisturizer

Scale Mean Level of Interpretation

Score Effectivenes
Range s
5 4.21- 5.00 Excellent Excellent: The product
Quality performs at a very high level
in this criterion.
4 3.41 – 4.20 Good Quality Very Good: The product
performs well, with some
minor issues or imperfections.

3 2.61 – 3.40 Acceptable Fair: The product has

Quality noticeable issues but still
functions adequately.
2 1.81 – 2.60 Below Poor: The product has
Acceptable significant flaws or issues but
still functions adequately.
1 1.00 – 1.80 Unacceptabl Very Poor: The product does
e Quality not meet expectations in this
area.

The following table presents a survey questionnaire designed to

assess synergy of Avocado oil, olive oil, and aloe vera extract based
on appearance, scent, texture, spreadability and Lightness.
The questions utilize a 5-point Likert scale where respondents will
rate each statement:
 1 = Very Poor,
 2 = Poor,
 3 = Neutral,
 4 = Good,
 5 = Exellent

Please answer all questions based on your personal experience and

preferences regarding the moisturizer made with avocado oil, olive
oil, and aloe vera gel. For each statement, kindly check the
number that best represents your level of agreement (1 = Very Poor

24
 to 5 = Exellent). Your responses will remain confidential and will be
used solely for the purpose of this study.

Table 2. Scoring Scheme for Sensory Evaluation.

Criteria/Rating
Exellen Goo Neutra Poor Very
t d l Poor
(2) (1)
(5) (4) (3)
Appearance
How visually appealing is the
moisturizer?
Does the product look fresh and well-
formulated?

Is the color of the product pleasing

and natural-looking?
Does the appearance meet your
expectations for an organic
moisturizer?
How would you rate the overall
appearance of the product?
Scent
How pleasant is the scent of the
moisturizer?
Is the scent too strong or too mild for
your preference?
Does the scent align with the concept
of an organic product?
How long does the scent last after
application?
Would you recommend this product
based on its scent?
Texture
How smooth is the texture of the
moisturizer?
Is the texture consistent throughout
the product?
Does the texture feel suitable for a
moisturizer?
How would you describe the
thickness of the product?
Does the texture enhance your
overall experience with the product?
Spreadability
How easily does the moisturizer
spread on the skin?

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Does the product absorb evenly when
applied?
Does the product require excessive
effort to spread?
Does the product leave residue after
spreading?
How satisfied are you with the
spreadability of the product?
Lightness
How light does the moisturizer feel on
your skin?
Does the product leave your skin
feeling greasy or oily?
Is the lightness suitable for an
organic moisturizer?
Does the product allow your skin to
breathe after application?
How would you rate the overall
lightness of the moisturizer?

After the sensory evaluation of organic moisturizer, the score sheets

were collected, recorded, tallied, summarized, and ready for
occupation. The mean was used to determine the level of
effectiveness, in terms of lightness, texture and scent. A t-test will
be performed to examine any significant differences or relationships
between variables. The basis of this scoring scheme is the five-point
Likert scale that was used in the evaluation.

Extracting of
Avocado, Olive
Preparation of and Formulating of
Materials Moisturizer Moisturizer

Data Analysis Sensory Testing of

Evaluation Moisturizer
26
Figure 4. Flow of Gathering of Materials and Methods of the Study

Research Ethics
The research will adhere to the standards of ethics set, with
full protection and well-being ensured for all respondents involved.
Each of the participating respondents will be held liable to sign an
informed consent before participation in this research. Participants
will be enlightened about the objectives, methods, and risks
associated with the use of the natural moisturizer. Participants will
be told that participation is undertaken voluntarily and that
withdrawal at any moment without repercussions is permitted.
All participant data is kept confidential by the investigators by
anonymizing their personal information for individual privacy
protection. In addition, in order to ensure the safety of the
participants, a preliminary skin patch test will be conducted to
detect allergic responses. When there is a compounded adverse
reaction to the skin in the course of the study, medical assistance
would be provided urgently. The study will be submitted for review
and approval before an ethics review board to ensure that all
procedures in the study meet high ethical standards and respect the
rights of participants appropriately during the course of the
investigation.
References
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Barrier disruption, dehydration and inflammation:
Investigation of the vicious circle underlying dry skin.
International Journal of Cosmetic Science, 43(6), 729–
737.https://doi.org/10.1111/ice. 12748

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Draelos, Z. D. (2018). The science behind skin care: Moisturizers.
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