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    NBFGR

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    joshigauta
    The document summarizes research conducted on induced breeding of two threatened fish species - Labeo dyocheilus and Labeo dero. Key findings include: 1) Broodstock of both species were collected from local rivers and reared in earthen ponds. 2) Induced breeding trials using ovaprim hormone were conducted in circular hatcheries, resulting in the production of fertilized eggs. 3) Hatching success rates ranged from 62-97% for L. dyocheilus and 52-89% for L. dero, with yolk absorption occurring within 56-64 hours.

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    NBFGR

    Uploaded by

    joshigauta
    48 views33 pages
    The document summarizes research conducted on induced breeding of two threatened fish species - Labeo dyocheilus and Labeo dero. Key findings include: 1) Broodstock of both species were collected from local rivers and reared in earthen ponds. 2) Induced breeding trials using ovaprim hormone were conducted in circular hatcheries, resulting in the production of fertilized eggs. 3) Hatching success rates ranged from 62-97% for L. dyocheilus and 52-89% for L. dero, with yolk absorption occurring within 56-64 hours.

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    NBFGR-

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    The document summarizes research conducted on induced breeding of two threatened fish species - Labeo dyocheilus and Labeo dero. Key findings include: 1) Broodstock of both species were collected from local rivers and reared in earthen ponds. 2) Induced breeding trials using ovaprim hormone were conducted in circular hatcheries, resulting in the production of fertilized eggs. 3) Hatching success rates ranged from 62-97% for L. dyocheilus and 52-89% for L. dero, with yolk absorption occurring within 56-64 hours.

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    Attribution Non-Commercial (BY-NC)
    Download as PPT, PDF, TXT or read online from Scribd
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    48 views33 pages

    NBFGR

    Uploaded by

    joshigauta
    The document summarizes research conducted on induced breeding of two threatened fish species - Labeo dyocheilus and Labeo dero. Key findings include: 1) Broodstock of both species were collected from local rivers and reared in earthen ponds. 2) Induced breeding trials using ovaprim hormone were conducted in circular hatcheries, resulting in the production of fertilized eggs. 3) Hatching success rates ranged from 62-97% for L. dyocheilus and 52-89% for L. dero, with yolk absorption occurring within 56-64 hours.

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    of 33

    vkuqoaf”kd laj{k.

    k ,oa Ák—frd ty iz{ks=ksa


    esa la[;k o`f) gsrq nks ”khr tyh; eNfy;ksa,
    dkyh jksgw rFkk eksg, ds iztudksa dk rkykc
    ds c¡/ks ty eas ikyu ,oa cht mRiknu

    vkbZ0 ts0 flag ,oa vthr flag


    ekfRL;dh thofoKku foHkkx
    eRL; foKku egkfo|ky;
    Xksk0 c0 iar —f"k ,oa izkS|ksfxdh egkfo|ky;]
    iUruxj ¼m/ke flag uxj½ mŸkjkapy
    Fish – Labeo dyocheilus and Labeo dero
    • Both are important food fish inhibiting upland
    streams and rivers at a mean sea level (MSL) of
    400-800m in lower Himalayan region of
    Uttaranchal

    • Locally L. dyocheilus is known as Kali, Kali rohu


    or Khadd and L. dero is known as Moh

    Order - Cypriniformes
    Family - Cyprinidae
    Sub family – Cyprininae
    Status
    • Vulnerable fish Dubey, 1994 and Prasad, 1994

    • Vulnerable fish - Perspective Plan Document


    (VISION 2020) of National Bureau of Fish
    Genetic Resources (NBFGR), Lucknow

    • Threatened Desai, 1994


    Reasons for loss of fish populations

    • Indiscriminate exploitation

    • Habitat loss

    • Pollution
    Strategies for fisheries management

    • Catch regulation

    • Habitat restoration

    • Stock supplementation
    Conservation strategies

    • In-Situ – in natural habitat

    • Ex-Situ –
    1. Cryopreservation
    2. Maintaining stock in captivity
    Objective

    • Collection of broodstock from nature & rearing in


    captivity to full maturity

    • Seed production through induced breeding in


    captivity
    Collection Site

    • The adult specimens of L. dyocheilus and L. dero


    were collected from Marchula, Domunda (Ram
    ganga river) and Kosi barrage, Tiger top (Kosi
    river)

    • Collection year 2000 - 2004


    River bed Kosi
    Ram Nagar Barrage
    Rearing and Feeding
    • Adults collected from nature were brought in live
    condition and were reared in earthen ponds at
    College of Fisheries, Pantnagar

    • Regular water supplementation through borewell


    water feeding

    • Feeding with conventional fish feed (oilcake and


    rice polish, 1:1) @ 5% body weight, twice daily
    Observation on GSI, CF and SCF of adult L. dyocheilus
    collected from wild during 2003
    Months Parameters No. of Male No. of Female
    fish fish
    Range Average Range Average

    May, 2003 Body weight (g) 04 310 – 720 570 04 710 – 1100 787.5

    Body length (cm) 30.0 – 41.0 36.7 37.0 – 46.7 41.3

    Gonad weight (g) 3.176 – 7.673 4.00 23.430 – 65.783 47.543

    GSI 0.857 – 1.065 0.997 3.082 – 7.872 6.175

    CF 1045 – 1204 1119 1069 – 1145 1099

    SCF 1034 – 1194 1158 985 – 1068 1031

    June, 2003 Body weight (g) 01 45 45 03 65 – 105 88.3

    Body length (cm) 14.5 14.5 17.0 – 22.0 20.0

    Gonad weight (g) 0.230 0.230 5.230 – 13.310 9.612

    GSI 0.511 0.511 8.555 – 12.676 10.698

    CF 1476 1476 986 – 1323 1112

    SCF 1469 1469 861 – 1210 995


    Data on GSI, CF and SCF of adult L. dero collected from nature
    in 2003
    Months Parameters No. Male No. of Female
    of fish
    fish Range Average Range Average

    May, Body weight (g) 02 650 – 760 705 02 710 – 730 720
    2003
    Body length (cm) 39.0 – 42.0 41.0 41.0 – 42.0 41.5
    Gonad weight (g) 6.693 – 7.117 6.905 88.976 – 90.088 89.532
    GSI 0.936 – 1.029 0.982 12.188 – 12.688 12.438
    CF 1026 – 1096 1061 958 – 1059 1009
    SCF 1016 – 1084 1050 837 – 930 883
    June, Body weight (g) 01 500 500 04 340 – 625 493.7
    2003
    Body length (cm) 39.0 39.0 32.0 – 37.0 34.6
    Gonad weight (g) 7.941 7.941 46.172 – 74.131 64.572
    GSI 1.588 1.588 11.860 – 14.412 13.215
    CF 843 843 1038 – 1234 1169
    SCF 830 830 897 – 1088 1014
    Brooders of L. dyocheilus
    Broodstock selection & Acclimatization

    Male – Rough Pectoral fin & oozing out milt


    after gentle pressure on belly

    Female – Pectoral fin soft, bulging & soft


    belly

    Brooders acclimatized for 3-4 hrs before induction


    Induced Breeding of L. dyocheilus and L. dero

    • Inducing hormone - Ovaprim

    • Induced breeding trials were conducted in circular


    hatchery at college of Fisheries, Pantnagar
    Details of induced breeding trials of L. dyocheilus
    S. Sex Range of Dose of Total No. Range of Avg. dia Fertilization Hatching Water
    No. Weight of ovaprim of eggs ova dia of eggs (%) (%) temperature
    fish (g) (ml/kg) released (mm) (mm) (0C)
    (Lakh)
    Breeding trial inYear 2001
    1. Female 600 - 1000 0.6 4.30 3.0-5.0 4.1 95.0 97% 25.0-28.5

    2. Male 400 - 950 0.4 & 05


    Breeding trials in year 2002
    1. Female 1200 - 1250 0.6 & 0.7 6.25 2.7-4.8 4.6 84.0 62.0 24.5 – 33.0

    2. Male 750 - 1000 0.4

    Breeding trials in Year 2003


    1. Female 450 - 1350 0.6 & 0.7 6.29 3.6-4.3 4.0 76.3 69.7 28.0 32.5

    2. Male 750 0.4


    Breeding trials in 2004
    1. Female 500 - 1350 0.6 4.81 2.9 – 4.1 3.4 91.4 76.0 24.5 – 26.0

    4. Male 300 -1000 0.4


    Details of Latency period, egg released,
    Hatching & Yolk resorption time in
    different breeding trials of L. dyocheilus
    Year/ Trial Latency Number of Average Yolk sac
    period (hrs) egg/kg fish hatching Time Absorption
    (hrs) (hrs)
    2001 1st 7.30 268750 15.00 60-62
    2002 1st 8.00 255000 14.30

    2002 2nd 7.30 255488 12.45

    2003, 1st 8.30 97500 15.30 60

    2003, 2nd 10.00 259259 15.00

    2003, 3rd 9.00 360000 14.00

    2004, 1st 9.30 174727 15.00


    Details of induced breeding trials of L. dero
    S. Sex Range of Dose of Total No. Range of Avg. dia Fertilization Hatching Water
    No. Weight of ovaprim of eggs ova dia of eggs (%) (%) temperature
    fish (g) (ml/kg) released (mm) (mm) (0C)
    (Lakh)
    Breeding trial inYear 2002
    1. Female 500 0.8 1.71 3.1 – 4.0 3.9 52.0 64.2 26.0-28.0
    2. Male 150 - 300 0.4
    Breeding trials in year 2003
    1. Female 275 - 600 0.8 & 0.9 3.32 4.8 – 5.7 5.0 72.2 67.6 25.5 – 31.5

    2. Male 200 - 450 0.6 & 0.7


    Breeding trials in Year 2004
    1. Female 460 -480 0.8 & 0.9 2.85 3.2 – 4.3 3.6 89.2 63.6 25.0 – 28.5
    2. Male 260 - 510 0.4
    Details of Latency period, egg released, Hatching & Yolk
    resorption time in different breeding trials of L. dero

    Year/ Trial Latency Average hatching Yolk sac


    period (hrs) Time (hrs) Absorption
    (hrs)
    2002 1st 11 - 17 14.30 58 - 64
    2003 1st 9.00 15.30
    200 2nd 10.30 15.00
    2003, 3st 9.30 14.00 56 - 63
    2004 9.00 13.30
    Mature Female L. dyocheilus
    Fertilized eggs of L. dyocheilus
    Comma shape embryo of L. dyocheilus
    Start of hatching of L. dyocheilus
    Hatchling of L. dyocheilus
    Fingerlings of L. dyocheilus
    Adult Labeo dyocheilus
    Fertilized eggs of L. dero
    Comma shape embryo of L. dero
    Start of hatching of L. dero
    50% hatching of L. dero
    One week hatching of L. dero

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