The Benthic Microbial Fuel Cell - Spectra 2014

NRL FEATURES
Fuel Cell
A benthic microbial fuel cell
(BMFC) is an oceanographic
power supply that can generate power indefinitely. The
BMFC sits at the sediment/water

(benthic) interface of marine environments, where it generates electrical
power using organic matter naturally
residing in marine sediments as its
fuel, and oxygen in overlying water
as its oxidant. At the Naval Research
Laboratory (NRL), we are developing
BMFCs to power persistent, in-water
intelligence, surveillance, and reconnaissance capabilities presently limited in operational lifetime by battery
depletion.
Thousands of battery-powered sensors are deployed each year that
provide valuable scientific information about marine environments. The
20
prospect of using BMFCs to power

these sensors indefinitely, or at least
far longer than possible with batteries,
is enticing we can acquire long-term
uninterrupted data and significantly
reduce the cost and logistics burden
of keeping sensors running. A BMFC
is maintenance free and nondepleting: the organic matter and oxygen
are constantly replenished by naturally occurring diffusion and advection; the electrode catalysts consist
of self-forming biofilms comprised of
microorganisms naturally inhabiting
the benthic interface; and there are
no moving, degradable, or depletable
components.
NRL has been a leader in developing
the BMFC and optimizing it for Navy
operational use. We have conducted
laboratory and field research to study
the microbial activity underlying
power generation: to determine the

effects of different oceanographic
and biogeochemical parameters, to
pair prototype BMFCs with sensors,
and to develop easily deployable
configurations. We have successfully
powered many useful devices with
small-scale BMFCs (less than 0.1
watt continuous output), and have
just begun development of full-scale
BMFCs (greater than 1 watt continuous output).
Early Laboratory Experiments
The bottom sediment of many
marine environments is reductant
enriched, due to metabolic activity
of sediment-dwelling microorganisms, whereas the overlying water
is oxidant enriched due to supply of
oxygen from the atmosphere. This
transition is referred to as the benthic
redox gradient. As a result of this
SPECTRA
NRL FEATURES
The combined power from six small-scale BMFCs was used to persistently operate a
meteorological buoy (center) in the Potomac River. The buoy measured local weather
conditions and transmitted data every five minutes to a land-based receiver.
gradient, when an inert electrode is

stepwise inserted into such sediments from overlying water, its open
circuit potential shifts by as much as
0.8 volts.
Taking advantage of this natural voltage gradient at the sediment/water
interface, my colleagues and I set
out to create a battery for powering
oceanographic sensors by embedding one electrode into reductantenriched marine sediment, where it
would act as an anode, and placing the other in oxidant-enriched
overlying water, where it would act
as a cathode. Our first experiments
involved a benchtop shoeboxsized aquarium containing marine
sediment, seawater, and a platinum
anode and platinum cathode, which
generated miniscule current across a
resistive load. The notion at the time
was that the anode was oxidizing
microbial-generated reductants in the
sediment (such as sulfide), and the
cathode was reducing oxygen in the
overlying water. Since the net reaction is thermodynamically favorable
(electron transfer from a reductant
to an oxidant, just like in a battery or
fuel cell), it was reasonable to expect that power could be expended
across a resistive load connecting
the electrodes as long as oxygen was
WINTER 2014
replenished at the cathode, and mass

transport (assumed to be diffusion)
supplied the anode reactants and
removed the anode products. A most
interesting result was that current
increased over time to a steady-state
level. In most electrochemical experiments, current decreases over time
due to depletion of the reactant and/
or diminished catalytic activity of the
electrode. I remember being dumbfounded watching the current rise.
We immediately followed with another
benchtop experiment using graphite
electrodes for the anode and cathode,
resulting in a significantly higher current density. After the system generated power for some time across a
resistor, we removed the anode from
the sediment and examined it to find
a biofilm adhering to its surface a
coating comprised of a community of
microorganisms from the sediment. A
genetics-based analysis of the biofilm
indicated that it was enriched in a
specific class of microorganisms, Deltaproteobacteria. The microorganism
available in pure culture most similar
to the microorganism enriched on the
BMFC anode was Desulfuromonas
acetoxidans, a dissimilatory metalreducing bacteria (DMRB) found in
marine sediment that couples oxidation of sedimentary acetate with
reduction of insoluble iron oxide

mineral deposits. Such microorganisms are fascinating (especially to
an electrochemist like me) for their
ability to transport respired electrons from inside the cell to insoluble
oxidants residing outside the cell
(referred to as extracellular electron
transport), acquiring a small amount
of energy for themselves in the process. It is thought that D. acetoxidans
in a BMFC anode biofilm oxidizes
organic matter in marine sediment
and transports the acquired electrons
to the underlying anode as if it were a
mineral deposit, effectively catalyzing
the anode reaction.
Early Field Deployments
In one of our first field experiments,
at the Rutgers University Marine Field
Station in the Great Bay, New Jersey in 2002, a BMFC with graphite
electrodes generated power continuously for nine months without indication of depletion in power before the
experiment was terminated. This and
subsequent longer-term field and
laboratory experiments revealed an
important attribute of microbial anode catalysts: as living entities metabolically benefiting from the reaction
they catalyze, they self-maintain
themselves and do not degrade over
time as long as their environment
21
NRL FEATURES
is hospitable. In this way, they can
maintain catalytic activity indefinitely.
Analysis of the anode biofilm of the
New Jersey BMFC indicated enrichment not only of D. acetoxidans, but
also of microorganisms in the Desulfobulbus or Desulfocapsa genera that
oxidize and disproportionate sulfur.
In sulfide-enriched sediment such as
the New Jersey site, a graphite anode can develop a passivating sulfur
precipitate on its surface that can
shut down current. It is thought that
these microorganisms act to clear the
sulfur from the anode surface, forming sulfate and sulfite, both soluble.
The next BMFC field experiments
were performed in 2003, at 1000
meters depth in the Monterey Canyon off the coast of California on a
cold seep, a fissure on the seafloor
effusing organic-rich water. We had
hypothesized that the high mass
transport rate of organic matter
would benefit BMFC power generation and we were correct. This
BMFC, equipped with a spear-like
graphite anode inserted vertically
into the seep, generated significantly
more power per unit footprint area
and geometric surface area of the
anode compared to our earlier experiments. This was exciting because
it demonstrated that placement of a
BMFC in an area with a high rate of
organic matter mass transport, such
as a subliming methane hydrate outcrop, could result in very high power
output, which could be of great use
to the Navy.
We next deployed a BMFC in 2004 in
the Potomac River off the NRL pier.
Here, the BMFC consisted of graphite electrode slabs attached with zip
ties to the top and bottom of milk
crate spacers that were positioned
on the river bottom with one electrode in the mud and the other in the
overlying water. An attached buoy
floating on the water surface measured air temperature, pressure, relative humidity, and water temperature,
and transmitted this data to my office
every five minutes. The entire buoy
including the radio transmitter was
22
powered by the BMFC. This required

a novel solution to convert the low
DC voltage output of the BMFC from
0.35 volts (dictated by the benthic
redox gradient) to 6 volts to charge a
capacitor, which in turn was used to
power the buoy. The capacitor was
needed to buffer the low but steady
power output of the BMFC with the
duty cycle of the buoy, whereby
nearly 99% of energy consumption by
the buoy occurred during the fraction
of a second during which the data
was transmitted. Between transmissions, the BMFC recharged the
capacitor. This was the first time that
a BMFC was used as a free-standing
power supply. The buoy ran flawlessly
for seven months. The river froze that
winter, trapping the buoy in ice, but
it kept running until the ice thawed
enough that large pieces began to
flow down river, dragging the buoy
and severing the mooring line connecting it with the BMFC. I got a call
from the Coast Guard who found the
buoy resting against a pier of the Wilson Bridge. (Lesson: always put your
phone number on any oceanographic
instrument you want back.)
A Practical Power Supply
Since 2004, a number of researchers
have been working to develop BMFCs into practical power supplies that
are straightforward to deploy. I am
fortunate to pursue this concept with
my NRL colleagues Dr. Jeff Book, Mr.
Andy Quaid, and Mr. Justin Brodersen, oceanographers; Dr. Yoko Furukawa, marine geochemist; and Dr. Jeff
Erickson and Mr. Marius Pruessner,
engineers; with funding from the Office of Naval Research and NRL. We
have deployed small-scale BMFCs in
coastal locations including California,
Gulf of Mexico, Maine, New Jersey,
North Carolina, and the Adriatic Sea.
These BMFCs have operated over
durations ranging from less than six
months to more than two years without indication of depletion in power
output. They have powered a hydrophone with a radio transceiver link, an
acoustic modem, and a surveillance
camera with a cellular link.
We are working to optimize the BMFC

design to maximize power output
while making anode embedment a
simple and reliable procedure. Maximizing power involves anode designs
that expose as much mass-transportaccessible surface area to sediment
as possible within mass and volume
constraints of the intended deployment platform, all while being easy
to properly embed into sediment. We
have been making steady progress,
hampered at times by the vagaries of
field research: in 2012, all our measurement equipment and 26 BMFCs
deployed at a New Jersey site were
lost when directly hit by Hurricane
Sandy.
In 2013, we began development of
full-scale BMFCs in NRLs newly
opened Laboratory for Autonomous
Systems Research (LASR), where we
have assembled a six-meter-diameter,
8000-gallon benthic mesocosm,
essentially a large indoor aquarium
filled with sediment and seawater to
replicate the benthic interface. This facility enables full-scale BMFC testing
in a controlled environment, greatly
reducing the cost and risk of BMFC
design development compared to field
testing. We have already made seven
deployments of a two-meter-diameter,
900-kilogram oceanographic mooring
equipped with various BMFCs to test
new designs. The LASR mesocosm is
greatly compressing the BMFC development horizon and I feel we are on
track to transition the BMFC in 2015.
By Leonard Tender
NRL Center for Bio/Molecular Science and
Engineering
SPECTRA
NRL FEATURES
BMFC graphite bottlebrush cathodes.
Dr. Leonard Tender (above, and with Marius

Pruessner at right) working in the benthic
mesocosm in the Laboratory for Autonomous
Systems Research. The mesocosm contains 18
inches of seawater and 24 inches of synthetic
marine sediment that has been inoculated with
real marine sediment to provide necessary
microbial activity. On the water surface are BMFC
graphite bottlebrush cathodes; when deployed in
the field, these cathodes are beneath the water
surface but above the sediment surface. The
vertical tubes indicate the locations of BMFC
anodes embedded in the sediment.
WINTER 2014
The benthic mesocosm in the Laboratory for

Autonomous Systems Research.
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NRL FEATURES

BMFC sits at the sediment/water

prospect of using BMFCs to power

power generation: to determine the

gradient, when an inert electrode is

replenished at the cathode, and mass

reduction of insoluble iron oxide

powered by the BMFC. This required

We are working to optimize the BMFC

BMFC graphite bottlebrush cathodes.

Dr. Leonard Tender (above, and with Marius

The benthic mesocosm in the Laboratory for

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